Review Article
Genome Editing in Plants: An Overview of Tools and Applications
Table 2
Examples of application of genome editing technology in plants.
| Number | Purpose or traits studied | Plant species | Method used | References | Year |
| 1 | Gene modification | Arabidopsis thaliana | ZFN | Osakabe et al. [15] | 2010 | 2 | Gene modification | Arabidopsis thaliana | ZFN | Zhang et al. [16] | 2010 | 3 | Gene editing | Nicotiana tabacum | ZFN | Townsend et al. [17] | 2009 | 4 | Targeted integration into endogenous loci | Zea mays | ZFN | Shukla [18] | 2009 | 5 | Targeted mutagenesis | Arabidopsis thaliana | TALEN | Christian et al. [19] | 2013 | 6 | Targeted mutagenesis | Oryza sativa | TALEN | Zhang et al. [20] | 2016 | 7 | Gene editing | Oryza sativa | TALEN | Li et al. [21] | 2012 | 8 | Targeted transcriptional gene repression | Arabidopsis thaliana | TALEN | Mahfouz et al. [22] | 2012 | 9 | Herbicide resistance | Arabidopsis thaliana | ODM | Sauer et al. [7] | 2016 | 10 | Herbicide resistance | Nicotiana tabacum | ODM | Sauer et al. [7] | 2016 | 11 | Herbicide resistance | Oryza sativa | ODM | Sauer et al. [7] | 2016 | 12 | Herbicide resistance | Zea mays | ODM | Sauer et al. [7] | 2016 | 13 | Herbicide resistance | Brassica napus | ODM | Sauer et al. [7] | 2016 | 14 | Gene knockout or editing | Arabidopsis thaliana | CRISPR/Cas | Li et al. [8] | 2013 | Mao et al. [13] | 2013 | 15 | Gene knockout or editing | Nicotiana benthamiana | CRISPR/Cas | Li et al. [8] | 2013 | 16 | Gene knockout or editing | Nicotiana tabacum | CRISPR/Cas | Gao et al. [23] | 2015 | 17 | Gene knockout or editing | Oryza sativa | CRISPR/Cas | Mao et al. [13] | 2013 | 18 | Multiplex genome editing | Arabidopsis thaliana | CRISPR/Cas | Li et al. [8] | 2013 | Mao et al. [13] | 2013 | 19 | Multiplex genome editing | Nicotiana tabacum | CRISPR/Cas | Cong et al. [24] | 2013 | Gao et al. [23] | 2015 | 20 | Gene insertion or replacement | Arabidopsis thaliana | CRISPR/Cas | Mao et al. [13] | 2013 | 21 | Gene insertion or replacement | Nicotiana benthamiana | CRISPR/Cas | Li et al. [8] | 2013 | 22 | Epigenetic gene regulation | Arabidopsis thaliana | CRISPR/Cas | Puchta [25] | 2016 | 23 | Targeted mutagenesis | Glycine max | CRISPR/Cas | Jacobs et al. [26] | 2015 | 24 | Targeted mutagenesis | Oryza sativa | CRISPR/Cas | Xu et al. [27] | 2016 | 25 | Improvement of nutritional value | Camelina sativa | CRISPR/Cas | Jiang et al. [28] | 2017 | 26 | Yield improvement | Solanum lycopersicum | CRISPR/Cas | Soyk et al. [29] | 2017 | 27 | Yield improvement | Zea mays | CRISPR/Cas | Peng et al. [30] | 1999 | 28 | Salt resistance | Oryza sativa | CRISPR/Cas | Osakabe et al. [31] | 2016 | 29 | Resistance to drought | Oryza sativa | CRISPR/Cas | Shan et al. [32] | 2013 | 30 | Herbicide resistance | Zea mays | CRISPR/Cas | Svitashev et al. [33] | 2015 | 31 | Biotic stress | Triticum aestivum | CRISPR/Cas | Wang et al. [34] | 2014 | 32 | Biotic stress | Oryza sativa | CRISPR/Cas | Li et al. [21] | 2012 | 33 | Biotic stress | Arabidopsis thaliana | CRISPR/Cas | Ji et al. [35] | 2015 | Nicotiana benthamiana | Ali et al. [36] | 2015 | 34 | Biotic stress | Nicotiana benthamiana | CRISPR/Cas | Baltes et al. [37] | 2015 | 35 | Biotic stress | Oryza sativa | CRISPR/Cas | Liu et al. [38] | 2012 | 36 | Biotic stress | Oryza sativa | CRISPR/Cas | Wang et al. [39] | 2016 | 37 | Biotic stress | Cucumis sativus | CRISPR/Cas | Chandrasekaran et al. [40] | 2016 |
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