Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

Table 2

System suitability parameters for the determination of the atenolol and trimetazidine in pure form/human urine.

Parameters	Atenolol	Trimetazidine	Reference value [39, 40]
Retention time ()	3.72/4.32	4.31/5.79	-----
Number of theoretical plates (N)	13211/2896	13935/13668	>2000
			increase with efficiency of separation
Tailing factor (T)	0.97/1.85	0.71/1.11	≤2
Capacity factor “Mass distribution ratio” (K′)	2.44/2.32	2.98/3.46	1-10 acceptable
Height equivalent to one theoretical plate (HETP)	0.01/0.04	0.01/0.01	The smaller the value, the higher the column efficacy
Resolution	1.62/4.89		>1.5
			Good separation between peaks of interest.
Selectivity factor (α)	1.11/1.49		> 1