Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation

Figure 2

Immunocytochemical detection of DSP and DMP-1 odontogenic markers. Upper panel: the staining pattern in positive controls for DSP (placenta), DMP-1 (mammary gland), and dental pulp tissue positive for both markers. Mesenchymal cells are negative for these markers. Lower panel: DSP and DMP-1 staining pattern in OLC differentiating cultures for 7, 14, and 21 days and reseeded expanded cells (EXP-21) at 7, 14, and 21 days after differentiation. Note the cytoplasmic, nuclear, and late extracellular DSP staining pattern (OLC-21 and EXP-21, left panel). DMP-1 is expressed in both nuclei and cytoplasm and also in extracellular space as matrix vesicles (OLC-21 and EXP-21, right panel). Bar correspond to 100 μm.