Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation

Figure 4

Cell growth curves. The hDPSC, OLC, and EXP-21 cells were detached daily for 21 days to determine the average number of cells in each cell population. hDPSC were maintained in culture medium without differentiation factors for 21 days, the OLC were stimulated with differentiation medium, and EXP-21 cells were maintained with differentiation medium for additional 21 days after detaching. Data are shown as mean ± SD and are based on three independent experiments and each was quantitated by triplicate ().