Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation

Figure 8

Extracellular matrix mineralization. (a) Mineralization was determined by alizarin red (upper panel) and von Kossa staining (lower panel). The strong staining of matrix indicates the apparent formation of calcification nodes. Scale bar: 100 μm. (b) The ALP activity as early marker of differentiation was associated with progressive mineralization of the extracellular matrix. Data shown are the mean ± SD of six replicas of three independent experiments (). Asterisks show significant differences with respect to negative control (hDPSC) (). (c) Absorbance measurement of alizarin red stain extracted at different stages of differentiation. Data shown are mean ± SD of six replicas of three independent experiments ().

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