Myo-Inositol Safety in Pregnancy: From Preimplantation Development to Newborn Animals

Figure 2

Effect of myo-inositol medium supplementation on embryo development during the third and fourth days of in vitro culture. Completion of mid-to-late preimplantation development by embryos cultured in the absence (grey bars) or the presence (solid bars) of 10 mM myo-inositol. Embryos were scored 72–74 hours (a) and 96–98 hours (b) p.f. Bars represent the mean ± SEM of 7 independent experiments of the fraction of embryos at each indicated stage. ((a), (b)) N, myo-Ins− embryos (grey bars), 97; myo-Ins+ embryos (solid bars), 106. Exp. blast.: expanded blastocyst. Asterisks indicate difference between treatments calculated by test with Yates correction for continuity: (a) ; (b) . (c) Representative images of expanded and nonexpanded blastocysts observed at day 4 among myo-Ins− (1, 3) and myo-Ins+ (2, 4) embryos; magnification: 40x (1, 2); 80x (3, 4). Black bars, 100 μm. Panel (b) is modified from [38].

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