Myo-Inositol Safety in Pregnancy: From Preimplantation Development to Newborn Animals

Figure 3

Localization of phosphorylated Akt in preimplantation embryos at the morula and blastocyst stage, after continuous culture in the presence or absence of myo-Ins. ((a), (b)) morula stage embryos; ((c), (d)) blastocyst stage embryos. In each panel, from left to right: FITC, Hoechst 33343, merge. The same pattern was consistently observed in all embryos analyzed; panels show representative embryos. Statistical analysis on these embryos, reported in the text, was performed on at least three embryos at the morula and blastocyst stages in three independent experiments.

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