Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA
Figure 2
Specific chemical reactions for m3C and m5C detection. (a) Cleavage of m3C and unmodified uridine by hydrazine. (b) Deamination of unmodified cytosine by bisulfate; m5C is resistant for deamination. (c) Specific oxydation of 5-6 double C=C bond in methylated pyrimidines by , OsO4, and OsO4 derivatives.