Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA

Figure 4

Specific reagents for 2′-O-methylated residues in RNA. (a) Selective cleavage of nonmethylated ribose residues at alkaline pH. The 2′-O-methylated residues are resistant for such cleavage. (b) Periodate oxydation of cisdiol residues at the terminal 3′-ribose. (c) Acylation of ribose free 2′-OH by SHAPE reagents N-methylisatoic anhydride (NMIA) and 1-methyl-7-nitro-isatoic anhydride.

(a)

(b)

(c)