Bone Marrow-Derived Mesenchymal Stem Cells Repair Necrotic Pancreatic Tissue and Promote Angiogenesis by Secreting Cellular Growth Factors Involved in the SDF-1α/CXCR4 Axis in Rats

Figure 1

The SDF-1α/CXCR4 axis could promote the expressions of cellular growth factors in BMSCs and induce angiogenesis in vitro. ((a), (d), and (e)) The CXCR4 expression of BMSCs was significantly unregulated after being stimulated by combined SDF-1α protein, which could be inhibited by the CXCR4 receptor agonist, AMD3100. ((b)–(d), (f)-(g)) Recombined SDF-1α protein could significantly promote BMSCS expression of VEGF, ANG-1, HGF, and TGF-β and the effect could also blocked by AMD3100. Moreover, there was a dose-effect relationship between combined SDF-1α protein and CXCR4, VEGF, ANG-1, HGF, and TGF-β expressions. Data are expressed as mean ± SD (, , and for SDF-1α (10 ng/mL or 100 ng/mL) versus NC, ^†, ^††, and ^††† for SDF-1α (10 ng/mL or 100 ng/mL) versus SDF-1α (10 ng/mL or 100 ng/mL) + AMD3100 10 μg/mL). (h) BMSCs supernatant could significantly promoted angiogenesis, especially after being pretreated with SDF-1α and this increase was partly offset by AMD3100. Both VEGF siRNA and Ang-1 siRNA significantly weakened proangiogenic capacity of BMSCs. Data are expressed as mean ± SD (^## for NC versus PBS, , , and for SDF-1α (10 ng/mL or 100 ng/mL), Ang-1 siRNA, or VEGF siRNA versus NC) (NC, normal control, SDF-1α, stromal cell derived factor-1a, CXCR4, CXC chemokine receptor 4, VEGF, vascular endothelial growth factor, and ANG-1, angiopoietin-1).

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