Bone Marrow-Derived Mesenchymal Stem Cells Repair Necrotic Pancreatic Tissue and Promote Angiogenesis by Secreting Cellular Growth Factors Involved in the SDF-1α/CXCR4 Axis in Rats

Figure 3

There is similarity in the proliferation and CXCR4 expression between unlabeled and SPION-labeled BMSCs ([Fe³⁺] = 25 g/mL). (a) The Prussian blue staining shows that BMSCs were labeled by SPION successfully. (b) The MTT tests are showing that the growth activity of SPION-labeled BMSCs ([Fe³⁺] = 25 g/mL) slowed slightly compared with that of the unlabeled BMSCs, which was also significantly stronger than that of other SPION-labeled BMSCs ([Fe³⁺] = 50, 75, or 100 g/mL). Data are expressed as mean ± SD ( and for normal control versus SPION-labeled BMSCS ([Fe³⁺] = 100 g/mL), and for normal control versus SPION-labeled BMSCS ([Fe³⁺] = 75 g/mL), and and for normal control versus SPION-labeled BMSCS ([Fe³⁺] = 50 g/mL) at each corresponding time point). ((c)-(d)) CXCR4 expression was detected by immunofluorescence and western blotting and similar between unlabeled and SPION-labeled BMSCs (). (SPION, superparamagnetic iron oxide nanoparticles, BMSCs, bone marrow-derived mesenchymal stem cells).

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