Bone Marrow-Derived Mesenchymal Stem Cells Repair Necrotic Pancreatic Tissue and Promote Angiogenesis by Secreting Cellular Growth Factors Involved in the SDF-1α/CXCR4 Axis in Rats

Figure 6

Transplanted BMSCs could reduce severe acute pancreatitis (SAP), promoting the formation of tubular complexes and inhibiting systematic inflammatory response, being involved in the SDF-1α/CXCR4 axis. ((a), (b)) The H&E staining of pancreatic tissues (magnification, ×100) is showing that the edema, infiltration, and necrosis were significantly reduced in the SAP+BMSCs group compared with SAP and SAP+anti-CXCR4 BMSCs groups at postoperative days 1, 4, and 7, respectively. A large number of tubular complexes were also investigated in SAP+BMSCs group (as indicated by red arrow in a 400x magnified picture). Serum amylase activity was also significantly reduced in SAP+BMSCs group than in normal, SAP, and anti-CXCR4 BMSCs groups at postoperative days 1, 4, and 7, respectively. (c) The levels of serum proinflammatory cytokines are significantly lower in SAP+BMSCs than in SAP and SAP+anti-CXCR4 BMSCs groups. In contrast, the levels of serum anti-inflammatory cytokines are significantly higher in SAP+BMSCs than in SAP and SAP+anti-CXCR4 BMSCs groups. Data are expressed as mean ± SD (^# for sham versus normal, , , and for SAP versus Normal, ^†, ^††, and ^††† for SAP versus SAP+BMSCs, , , and for SAP+BMSCs versus SAP+anti-CXCR4 BMSCs). Data analysis was performed by Student’s test (BMSCs, bone marrow-derived mesenchymal stem cells, H&E, hematoxylin-eosin).

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