Abstract and Applied Analysis

Volume 2012 (2012), Article ID 590326, 13 pages

http://dx.doi.org/10.1155/2012/590326

## Analysis of Wave Solutions of an Adhenovirus-Tumor Cell System

^{1}Laboratoire des Interactions Ecotoxicologie, Biodiversité, Ecosystèmes, Université de Lorraine, CNRS UMR 7146, 8 rue du Général Delestraint, 57070 METZ, France^{2}Laboratoire de Mathématiques Raphaël Salem, Université de Rouen, UMR 6085 CNRS, Avenue de l'Université, P.O. Box 12, 76801 Saint Etienne du Rouvray, France

Received 2 December 2011; Accepted 13 February 2012

Academic Editor: Muhammad Aslam Noor

Copyright © 2012 Baba Issa Camara and Houda Mokrani. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

#### Abstract

We discuss biological background and mathematical analysis of glioma gene therapy for contributing to cancer treatment. By a reaction-diffusion system, we model interactions between gliom cells and viruses. We establish some sufficient conditions on model parameters which guarantee the permanence of the system and the existence of periodic solutions. Our study has experimental and theoretical implication in the perspective management strategy of therapy.

#### 1. Introduction

Diffuse infiltrative gliomas are the most frequent primary central nervous system (CNS) tumors in adults. Their deserved reputation as devastating diseases is due in large part to their widespread invasiveness in the neuropil, that is, the dense network of interwoven neuronal and glial cell processes. Partially because of their growth pattern, curative treatment for diffuse gliomas is generally impossible. Although patients with low-grade diffuse gliomas may survive for multiple years, these tumors lead to death of the patient sooner or later, often after progression to high-grade malignancy. Whereas surgery of most other tumors aims at complete resection, the diffuse growth of gliomas in the brain parenchyma precludes complete tumor removal. So to increase the length of survival time of patients with malignant brain tumors, novel therapeutic alternatives are currently being explored. Some of these experimental treatment strategies are based on advances in immunotherapy, stem cell therapy, local chemotherapy, and radiotherapy. However, in areas where the original tissue architecture is relatively preserved, the blood-brain barrier that may form an obstacle for optimal delivery of chemotherapeutics to diffuse infiltrative tumor cells eradicating diffuse infiltrative glioma cells by radiotherapy without significantly damaging the infiltrated brain parenchyma has been difficult to achieve [1].

In addition, gene therapy is becoming a promising alternative. In essence, gene therapy consists of the delivery of a gene of interest to tumor cell populations to control and, when possible, kill the growing tumor. Viruses are prominent vehicles for gene therapy, and some adenoviral vectors exhibit oncolytic properties. To this end, a variety of viral vectors have been developed, with oncolytic viruses emerging as an innovative therapeutic tool for these tumors. To be effective, a virus used for oncolytic therapy must have several features. The desired properties of these vectors include selectivity for the tumor target, minimal brain and systemic toxicities, and the capacity to penetrate and diffuse throughout the brain to reach all neoplastic foci residing beyond the resection border of the tumor. In addition, the viral vector needs to remain active despite evoking an immune response. The goal of developing an ideal vehicle for treatment of malignant brain tumors remains to be achieved. A wide variety of viral vectors have been developed and tested in the setting of gene therapy for malignant gliomas. These are based on different kinds of viruses, such as herpes simplex virus, retrovirus, measles virus, reovirus, and adenovirus. Some have shown promising results when tested in animal models of intracranial gliomas, but, to date, clinical trials performed in humans have not shown a significant increase in survival.

Various vectors have been targeted toward cancer cells by deleting the genes responsible for bypassing those cells’ antiviral proteins. Without these genes, the designed vectors will only be able to replicate within cancer cells with disrupted antiviral mechanisms. The deletion of viral genes to enhance specificity for the killing of neoplastic cells is a principle well exemplified by the actions of two oncolytic adenoviruses, ONYX 015 and Ad5-Delta24. ONYX 015 has a deletion in the viral genomic region coding E1B 55kd. This deletion effectively limits the replication of the virus to neoplastic cells that have a defective p53 pathway [2, 3]. A Phase I clinical trial to examine the effects of injection of ONYX 015 into peritumoral regions of recurrent malignant gliomas was recently completed and published [4]. In that study, ONYX 015 was injected into the walls of tumor resection cavities. This trial proved that the injection of up to 1010 plaque-forming units of ONYX 015 into brain tissue surrounding a resected malignant glioma is safe in humans.

Extensive efforts have been dedicated over many years to mathematical modelling of cancer development [5–7]. These mathematical models serve as valuable tools to predict possible outcomes of virus infection and propose the optimal strategy of anti-virus therapy. Wodarz [8, 9] presented a mathematical model that describes interaction between two types of tumor cells, the cells that are infected by the virus and the cells that are not infected but are susceptible to the virus so far as they have cancer phenotype and the immune system. Our system is more general than the one considered in [8, 9] even when there is no diffusion. Because the free virus particles are very small, they disperse in the fluid tissue like Brownian particles. Therefore, we have incorporated into our model a diffusion term for the free viruses. We also assume that the tumor has a logistic growth, which can be slowed down by the inhibitor, captured in the expression . Thus, the tumor admits a maximum size and density defined by the carrying capacity . When the virus is administered, the dynamic interactions between the virus and tumor cell populations are described by the following diffusive ratio-dependent predator-prey model of reaction-diffusion equations in the tumor region : where is the number density of susceptible and uninfected tumor cells, is the number density of infected tumor cells, and is the number density of free virus, that is, virus in the extracellular tissue. When parameters of system (1.1)–(1.4) are constant, we determined in [10] the conditions for optimal therapy and, estimated by numerical simulations, the patient survival time when tumor cannot be cured. This paper is organized as follows Section 2 is devoted to some preliminaries, which are needed in next sections, including some lemmas, due to Walter and Smith. In Section 3, some conditions for the ultimate boundedness of solutions and permanence of this system are established

#### 2. Preliminaries

We need the following lemmas due to Walter [11] and Smith [12], respectively.

Lemma 2.1. *Suppose that vector functions and , , satisfy the following conditions: *(i)*they are of class in , , and of class in , where is a bounded domain with smooth boundary;*(ii)*, where , , vector function is continuously differential and quasi-monotonically increasing with respect to and , , ;*(iii)*, .** Then, , for all .*

Lemma 2.2. *Assume that and are positive real numbers, a function is continuous on , continuously differential in , with continuous derivatives and on , and satisfies the following inequalities:
**
where is bounded on . Then on . Moreover, is strictly positive on if is not identically zero.*

Consider the following logistic differential equation: where ; and are positive constants.

Lemma 2.3. *Every solution , of (2.2) satisfies
*

#### 3. Permanence

Throughout the paper we always assume that : , , , , and are bounded positive-valued functions on continuously differential in and and are periodic in with a period .

*Definition 3.1. *Solutions of system (1.1)–(1.4) are said to be ultimately bounded if there exist positive constants *, **, * such that for every solution there exists a moment of time such that , , , for all and .

*Definition 3.2. *System (1.1)–(1.4) is said to be permanent if there exist positive constants and such that for every solution with nonnegative initial functions *, *, and *, **, **, **, *there exists a moment of time such that *, **, **, *for all and *. *

For simplicity, for a bounded function , we denote and . Now we have the following positively invariant principle for system (1.1)–(1.4).

Theorem 3.3. *Assume that conditions hold, then nonnegative and positive quadrants of are positively invariant for system (1.1)–(1.4).*

*Proof. *Suppose is a solution of system (1.1)–(1.4) with initial condition (), (), (). Let be a solution of
It holds that
which implies that is a lower solution of (1.1). By Lemma 2.2, we have , for all and . In addition, since (), then , for all and . Thus by Lemma 2.1, is bounded from below by positive function , and so .

Let be a solution of

It holds that
which implies that is a lower solution of (1.2).

Let be a solution of

It holds that
which implies that is a lower solution of (1.3).

A similar argument to leads to that and are bounded from below, respectively, by positive functions and .

Theorem 3.4. *Assume that conditions hold then all solutions of system (1.1)–(1.4) with nonnegative initial functions are ultimately bounded.*

*Proof. *Let be a solution of
where is such that .

It holds that
So, we get

Therefore, Lemma 2.1 gives .

Note that, according to the uniqueness theorem, the solution of (3.7) does not depend on for , and so satisfies the ordinary differential equation:
By Lemma 2.3, we have
So, there exists and such that .

For the infected tumor cells, we have the following inequality:
So, we get
Therefore, Lemma 2.1 gives , where satisfies the ordinary differential equation:
Since
thus, there exists and such that .

For the virus in the extracellular tissue, we have the following inequality:
So, we get
Therefore, Lemma 2.1 gives , where satisfies the ordinary differential equation:
Since
thus, there exists and such that .

Theorem 3.5. *Assume that conditions hold; in addition, if , then the system (1.1)–(1.4) is permanent.*

*Proof. *Theorem 3.4 implies that there exists such that , , and starting with a certain moment of time. Note that, by comparison principle, if (), (), and (), then , , and for all and . Considering the solution on the interval with some , we get separated from zero. Therefore, we can assume that , and, . Let be a solution of
So using the inequality
one has, by (3.20),
Therefore, Lemma 2.1 gives . By the condition , we have
Therefore, there exists such that for large enough.

Let be a solution of
Using the inequality
we have, by (3.24),
Therefore, , as . Therefore, there exists such that for large enough.

Now, let be a solution of
By the following inequality,
we have
Therefore, , as . Therefore, there exists such that for large enough.

#### 4. Periodic Solutions

Theorem 4.1. *Assume that conditions hold and system (1.1)–(1.4) is permanent. Moreover, if one assumes the following conditions
**
then the system has a unique globally asymptotic stable strictly positive -periodic solution.*

*Proof. *For convenience, we denote and similar meaning to , , , , and . Let and be two solutions of system bounded by constants and for below and above. Consider the function
So,
It follows from the boundary condition (1.4) that
The terms
Thus, we have
where is the maximal eigenvalue of the diagonal matrix with
So, we deduce that
Thus, , , and , as . By Theorem 3.3, solutions of system (1.1)–(1.4) are bounded in the space , where and . Therefore,
Consider the sequence
Then is compact in the space . Let be a limit point of this sequence. It follows, from
that .

Next, let and be two limit points of the sequence . Using (4.9) and , we have
Thus, , and so is the unique periodic solutions of system (1.1)–(1.4). By (4.9), it is asymptotically stable.

#### References

- A. Claes, A. J. Idema, and P. Wesseling, “Diffuse glioma growth: a guerilla war,”
*Acta Neuropathologica*, vol. 114, no. 5, pp. 443–458, 2007. View at Publisher · View at Google Scholar · View at Scopus - J. R. Bischoff, D. H. Kirn, A. Williams et al., “An adenovirus mutant that replicates selectively in p53-deficient human tumor cells,”
*Science*, vol. 274, no. 5286, pp. 373–376, 1996. View at Publisher · View at Google Scholar · View at Scopus - C. Heise, A. Sampson-Johannes, A. Williams, F. McCormick, D. D. Von Hoff, and D. H. Kirn, “ONYX-015, an E1b gene-attenuated adenovirus, causes tumor-specific cytolysis and antitumoral efficacy that can be augmented by standard chemotherapeutic agents,”
*Nature Medicine*, vol. 3, no. 6, pp. 639–645, 1997. View at Publisher · View at Google Scholar · View at Scopus - E. A. Chiocca, K. M. Abbed, S. Tatter et al., “A phase I open-label, dose-escalation, multi-institutional trial of injection with an E1B-attenuated adenovirus, ONYX-015, into the peritumoral region of recurrent malignant gliomas, in the adjuvant setting,”
*Molecular Therapy*, vol. 10, no. 5, pp. 958–966, 2004. View at Publisher · View at Google Scholar · View at Scopus - N. L. Komarova, “Mathematical modeling of tumorigenesis: mission possible,”
*Current Opinion in Oncology*, vol. 17, no. 1, pp. 39–43, 2005. View at Publisher · View at Google Scholar · View at Scopus - A. S. Novozhilov, F. S. Berezovskaya, E. V. Koonin, and G. P. Karev, “Mathematical modeling of tumor therapy with oncolytic viruses: regimes with complete tumor elimination within the framework of deterministic models,”
*Biology Direct*, vol. 1, article no. 6, 2006. View at Publisher · View at Google Scholar · View at Scopus - J. T. Oden, A. Hawkins, and S. Prudhomme, “General diffuse-interface theories and an approach to predictive tumor growth modeling,”
*Mathematical Models & Methods in Applied Sciences*, vol. 20, no. 3, pp. 477–517, 2010. View at Publisher · View at Google Scholar · View at Zentralblatt MATH - D. Wodarz, “Viruses as antitumor weapons: defining conditions for tumor remission,”
*Cancer Research*, vol. 61, no. 8, pp. 3501–3507, 2001. View at Google Scholar · View at Scopus - D. Wodarz and N. Komarova,
*Computational Biology of Cancer: Lecture Notes And Mathematical Modelin*, World Scientific, Singapour, 2005. - B. I. Camara, H. Mokrani, and E. Afenya, “Mathematical modelling of gliomas therapy using oncolytic viruses,” to appear.
- W. Walter, “Differential inequalities and maximum principles: theory, new methods and applications,” vol. 30, no. 8, pp. 4695–4711, 1997. View at Publisher · View at Google Scholar
- H. L. Smith, “Dynamics of competition,” in
*Mathematics Inspired by Biology*, vol. 1714 of*Lecture Notes in Math.*, pp. 191–240, Springer, Berlin, Germany, 1999. View at Google Scholar · View at Zentralblatt MATH