Critical Review on Plant Micropropagation of Ethiopian Plants Reported So Far: Existing Gaps, Required Standardization, and Future Research Direction

Table 1

List of plant species studied in Ethiopia, published and reported so far, culture vessels, in vitro growth and regeneration conditions, and nonnutritional components used.

List of species	Culture vessel used	Growing conditions	Nonnutritional components used	Ref.
Prunus salicina Lindl.	Magenta GA-7	T = 25 ± 2°C; LD = 16 h; LI = 37.8 μmol/m^2/s)	Agar (0.8%)	[37]
Vitis vinifera L.	Magenta GA-7	T = 27 ± 2°C; = 16 h; LI = 40 μmol/m^2/s);	Agar (0.7%)	[38]
Zingiber officinale Roscoe	Culture jar	T = 20–25°C	Agar (0.8%)	[39]
Guizotia abyssinica (L.f.) Cass.	Culture jar	T = 25 + 2°C; LI (40 μmol·m^−2·s^−1); For CI:- the dark at	Agar (0.7%)	[40]
Carica papaya L.	Magenta	T˚ = 25 ± 2°C; LD = 16 h; LI = 28–35 μmol/m^2/s; RH 70%	Agar (0.8%)	[27]
Saccharum officinarum L.	Glass culture jar	T = 25 ± 2°C LD = 16; LI = 35 μmol/m^2/s; RH = 75–80%	Agar (0.8%)	[42]
Ensete ventricosum (Welw.) Cheesman	CV = 250 ml jars	T = 27 ± 2°C; LD: 14 h LI: 40 μmol/m^2/s; RH: 60–70%	Agar (0.8%) & activated charcoal (0.3%)	[28]
Triticum aestivum L., Var.;Yerer, Simba, Ude and Galama,	Petri dish	T: 24 ± 4°C; LD = 16 h; LI = 19.09–28.80 μmol/m^2/s	Agar (0.8%)	[43]
Manihot esculenta Crantz.	Test tube	T = 22–23: LD:16 h; LI:14 μmol/m^2/s	Agar (0.8%)	[44]
Ananas comosus (L.) Merr.	Test tubes	T = 25°±2°C; LD = 16 h; LI = 28–42 μmol/m^2/s	Agar (0.8%); Enset starch (60 g/l) & agar (2 g/l) for rooting and phythogel 3 g/l	[45]
Sesamum indicum L.	Magenta GA7&Petri dish	Vials incubated under continuous light for rooting	Not mentioned	[46]
Coffea arabica L.	Magenta-GA7	T = 25°±2°C; LI = 40 μmol μmol/m^2/s; LD = 16 h	0.8% Agar	[11]
Cordeauxia edulis Hemsl.	Magenta	T: 25°±2°C, LD = 16 h	0.7% agar and Charcoal	[55]
Musa x paradisiaca L.	Test tube	T˚ = 25 ± 2°C: LD = 16 h cool white fluorescent	Agar (0.8%)	[47]
Echinops kebericho Mesfin,	Magenta jars	T = 25 ± 2 C; LD = 16 h; LI = 22 μmol/m^2/s	Agar (0.8%)	[13]
Capsicum annuum L.	Test tube	T = 27 ± 2°C, LD = 12 h; LI = 14–28 μmol/m^2/s	(0.7% agar-agar)	[23]
Coccinia abyssinica (Lam.) Cogn.,var. 29)	Tubes or jars	T = 25 ± 2°C	Agar (0.8% w/v)	[52]
Aframomum corrorima (Braun) Jansen,	100 ml baby food jar	T = 25 ± 2°C LD = 16 h; LI = 28 μmol/m^2/s	Agar-agar 0.7%	[49]
Solanum tuberosum L., var.Hunde and var. Ararsa	culture jar	T^◦ = 24°C LD = 16 h; LI = 35 μmol/m^2/s	Agar 0.8%	[35]
Ipomoea batatas (L.) Lam.,var. Beletech, Awassa-83 &Belela	Magenta jars	Grown under florescent light at 25 ± 2°C	Agar 0.6%	[53]
Brassica carinata A. Br., Holeta -1 and yellow dodola	Magnet box	T = 25 ± 2°C; LD: 16/8 h; LI: 37.8 μmol/m^2/s)	Agar 0.6%	[29]

g = gram, h = hours, l = liter, LD = light duration per day in hours, LI = light intensity in μmol/m²/s (= micromole per meter square per second), ml = milliliter, RH = relative humidity, s = seconds, T = temperature in degree Celsius,