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| Species studied | Objectives of the study | Results reported | Culture type | Ref. |
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| 1. Prunus salicina Lindl. | Micropropagation and virus cleaning | The best shooting was obtained on MS + 0.5 mg/l BAP in combination with 0.1 mg/L of IBA; similarly, the best rooting response was obtained on ½ MS media supplemented with 1.0 mg/L IBA | Micropropagation from nodal segment | [37] |
| 2. Vitis vinifera L. | Salt tolerant cultivar identification | The degree of NaCl tolerance varied among the cultivars evaluated | Shoot culture from shoots with a single node | [38] |
| 3. Zinger officinale Roscoe | Micropropagation & Virus cleaning | Better shooting was reported when MS was supplemented with (2 mg/l and 1 mg/l BAP; 7 shoots per ex plant was produced | Micropropagation from auxiliary bud and shoot tip | [39] |
| 4. Guizotia abyssinica (L.f.) Cass. | Indirect in vitro regeneration Virus cleaning | NAA (5 mg/l) + BAP (1 mg/l) gave better CI from hypocotyl ex plant (98.3%); the highest percentage of shoot formation was obtained when cotyledons were cultured on a medium supplemented with 3.0 mg/l IAA + 1.0 mg/l BAP. Maximum number of shoots per explant (20.3) was obtained from a medium containing 0.1 mg/l NAA + 1 mg/l BAP. Best shooting was on 0.5 mg/l IBA. Shoot survival in soil was 65% | CI from hypocotyl and cotyledon for in vitro shooting and rooting | [40] |
| 4. Carica papaya L. | Micropropagation | The highest mean, mean number of shoots, leaf, and shoot length were obtained on 1 mg/l BAP and 0.5 mg/l NAA. A minimum (9.5 days) and longer (13.5 days) for shoot initiation were recorded on BAP at 0.5 mg/l + 0.5 mg/l NAA and BAP at 2.0 mg/l + 0.5 mg/l NAA, respectively. Half-strength MS with 2 mg/L IBA is the most suitable treatment for root induction. The maximum number of roots (16.25) and root length (3.92 cm) were measured on shoots pretreated on MS media supplemented with 1.5 mg/l IBA. | Shoot bud culture for shoot initiation (micropropagation) | [27] |
| 5. Saccharum officinarum L. | Micropropagation and Virus cleaning | Genotype N52 showed a maximum of 6.95 ± 0.19 shoots per explant with 4.75 ± 0.06 cm shoot length and 5.65 leaves per shoot on liquid MS medium fortified with 2 mg/l BAP + 0.5 mg/l kinetin while genotype N53 produced a maximum of 6.30 ± 0.26 shoots per explant with 3.94 ± 0.03 average shoot length and 5.83 leaves per shoots on liquid MS medium supplemented with 1.5 mg/l BAP + 0.5 mg/l kinetin | Shoot tip culture for micropropagation | [42] |
| 6. Ensete ventricosum (Welw.) Cheesman | Micropropagation, virus cleansing | The minimum number of days for multiplication of shoots (11.6) and maximum shoot mass number of shoots (23.0) were obtained on 4.5 mg/l BAP and 1.5 mg/l for enset cultivar Mezia. The minimum number of days (10.5) for root induction was observed for the same cultivar on media with 1.5 mg/l IBA and the maximum root number (3.8) was recorded at 2 mg/l IBA | Shoot tip culture for micropropagation | [28] |
| 7. Triticum turgidum and Triticum aestivum L., Var.; Yerer, Simba, Ude & Galama | Micropropagation, somatic embryogenesis, and anther culture | Genotypes, types of media, concentrations of 2,4-D and Kin and durations of cold pretreatment at 4°C affected direct formation of embryonic tissues independently. Stage II of wheat spikes, MS medium containing 1 mg/l of each of 2,4-D and Kin and 15 days of cold pretreatment were found to be the best conditions for direct formation of embryonic tissues; the highest frequency of shoots was regenerated from the cultured embryonic tissues of variety Yerer (41.6%) and Simba (41.3%) on medium containing 0.1 mg/l 2,4-D; From a total of 14,524 cultured unfertilized ovaries, 1,100 embryonic tissues (7.6%) and 75 regenerants were obtained. The average percentage of embryonic tissues and regenerants were 9.0 and 1.1% from 3,444; 9.8 and 0.55% from 4,732; 5.6 and 0.17% from 2,988; 4.7 and 0.12% from 3,360 cultured unfertilized ovaries for varieties Yerer, Simba, Ude, and Galama, respectively | unpollinated ovary cultures for direct formation of embryogenic tissue and subsequent shooting | [43] |
| 8. Musa esculenta Crantz. | Micropropagation | BAP and Kin, For shooting, NAA and BAP for root induction augmented with 0.75 mg/l BAP and Kin gave the highest shooting (7.3/explant); NAA 0.5 mg/l gave the highest rooting (6.14 root/explant) | Auxilar nodal bud culture (micropropagion) | [44] |
| 9. Sesamum indicum L. | Micropropagation | The highest mean shooting (87.15%) was obtained on 0.25 mg/l IBA + 0.5 mg/l NAA. A survival rate of 66.7% and the highest shooting (38.33%) were obtained. The highest callusing was obtained with (2,4-D 2 mg/l and BAP (1 mg/l) with 56% callusing and callus weight of 8.33g | Anther culture | [46] |
| 10. Coffea arabica L. | Micropropagation | Leaf explants cultured on MS with 0.05 mg/l Kinetin in combination with 0.1 mg/l IBA resulted in embryonic callus. The highest number of embryos that germinated per ex plant (14.0 ± 1.7) was obtained on MS with 2.0 mg/l BAP in combination with 0.5 mg/l GA3. The maximum number of roots per plantlet (3.0 ± 1.0) was obtained on a 1/2 MS medium containing 0.5 mg/l IBA | Leaf explant culture for Embryogenic callus formation | [11] |
| 11. Cordeauxia edulis Hemsl. | Micropropagation&Optimization of sterilization protocol | 5% NaOCl for 10 m is the most effective in surface sterilization. The sterilized seed cultured on ½ B5 media was most suitable with a germination of 26.67%. The highest shoot initiation (89%) of explants produced shoots), the number of shoots per explant, and the number of leaves per shoot were obtained from cotyledonary node explants cultured on MS media supplemented with 2 mg/l BAP within nine weeks. But, the highest shoot length and shoot fresh weight were recorded from control (free BAP) and 6.00 after 42.57 ± 0.58 days of culture BAP, respectively. The highest shoot multiplication (4.56 number of shoot induced) and elongation (2.97 cm) were obtained from the induced shoot cut placed on MS media supplemented with 2.00 mg/l BAP + 6.00 mg/l GA3 and free BAP + 6.00 mg/l of GA3, respectively | cotyledon node explant | [55] |
| 12. Musa x paradisiaca L. | Micropropagation | Shoot initiation was greater on MS basal medium with 3 mg/l BAP for Dwarf and Giant Cavendish and 2 mg/l for Poyo varieties. BAP and IAA at 3 + 0.4, 4 + 0.4, and 3 + 0.2 mg/l for Dwarf, Giant, and Poyo, respectively, were better combinations for high rates of shoot proliferation and elongation. Further multiplication of shoots required up to 5 times subculturing of 1 month each on the same media combination. Better rooting was obtained when the shoots were cultured on MS medium with 2.12 mg/l (NAA) for Dwarf and Giant Cavendish and 1.74 mg/l (IBA) for Poyo | Meristem culture | [32] |
| 13. Echinops kebericho Mesfin | Micropropagation & Virus cleaning | 100% germination was recorded in fresh seeds and dropped to 65.18% and 22.3% for 3 and 5 months seeds, respectively. After 42.57 ± 0.58 days of culture with 1.0 Kin and 0.5 after 42.57 ± 0.58 days of culture Kin + 0.1 mg/l NAA showed maximum shoot proliferation on shoot induction media and shoot multiplication media, respectively. Better rooting was obtained on 1/3 MS containing 1.5 mg/l NAA with 8.23 roots and 4.82 cm root length and established under greenhouse with 83% survival | Micropropagation using shoot tips | [13] |
| 14. Capsicum annuum L. | Micropropagation, conservation Optimatizion of sterilizing protocol | 3% active chlorine for 20 m was found to be a better treatment combination yielding 82.5% ± 5.00% contaminant-free germinated seedlings. For shoot induction, MS + 4.5 mg/l B AP + 0.5 mg/l IAA and MS medium containing 8 mg/l Zeatin were found to be better, resulting in 77.5% ± 5.00% and 67.50% ± 5% induction percentage for nodal and shoot tip explants, respectively. Maximum shoot multiplication responses were obtained on MS + 3 mg/l BAP + 2 mg/l Kinetin with a mean number of 9.2 ± 0.2 and 8.6 ± 0.00 shoots for nodal and shoot tip explants respectively. Best shoot elongation and rooting responses were reported on MS + 0.5 mg/l IBA resulting mean value of 29.6 ± 0.12 root number, 4.25 ± 0.20 cm root length, and 5.12 ± 0.20 cm shoot height. The plantlets showed 77.5% survival during acclimatization and transplanting | Micropropagation (shoot apices and node) | [23] |
| 15. Coccinia abyssinica (Lam.) Cogn.var. 29 | Micropropagation and optimation of sterilants | NaOCl (2.0%) for 5 min gave high percentages of survived nodal (79.43 ± 0.6) and shoot tip (74.33 ± 0.58) explants. BAP (3.0 μM) was found to be an optimum concentration for SI, yielding 80% for nodal and 70% for shoot tip explants. The combination of BAP (3.0 μM) with IAA (0.5 μM) was reported to have been obtained as optimum concentration yielding 13.4 and 11.03 shoots per explants for nodal and shoot tip, respectively, for shoot multiplication. ½ MS with IBA (0.5 μM) and IAA (1.5 μM) yielded more than 90% rooting with optimum root number and length | Micropropagation (shoot tip) | [53] |
| 16.Aframomum corrorima (Braun) Jansen | Micropropagation | Cultures initiated from axillary bud explants of rhizome using 0.5 mg/l thidiazuron (TDZ) in combination with 3 mg/l paclobutrazol (PBZ) gave about 26 shoots/explant (about 12.6-fold than the control) within eight weeks. Shoot multiplication was also from rhizome enhanced when TDZ at 0.5 mg/l was simultaneously used with either 2 mg/l imazalil (IMA) or 3 mg/l N6-benzyladenine (BA) in the culture medium. Subsequent shoot elongation and development of functional roots were reported to have been attained after one to three monthly subcultures on a plant growth regulator (PGR)-free basal medium | Micropropagation | [49] |
| 17. Solanum tuberosum L.var. Hunde and var. Ararsa | Micropropagation and determination of the optimum concentration of sucrose for microtuberinduction | Two potato varieties were tested for their microtuber induction under five levels of sucrose (40, 60, 80, 100, and 120 g/l. In both varieties, among the five concentrations of sucrose, MS medium with 60 g/l sucrose exhibited a better response. This medium produced an average value of (1.97 ± 0.02) microtuber number, (3.60 ± 0.04 mm) microtuber diameter, and (0.08 ± 0.002 g) microtuber weight tuber in the variety after 42.57 ± 0.58 days of culture. On the other hand, after 35.67 ± 0.58 days of culture, the mean values of (2.90 ± 0.031) microtuber number, (2.95 ± 0.01 mm) microtuber diameter, and (0.06 ± 0.001 g) microtuber weight were recorded in the variety Hunde | Micropropagation (auxiliary bud) | [35] |
| 18. Ipomoea batatas (L.) Lam. var. Beletech, Awassa-83 & Belela | Micropropagation | The minimum days for root induction was recorded for variety Beletech (3.167 days) in shoots cultured on media supplemented with 0.5 mg/l IBA with 0.5 mg/l NAA. Variety Awassa-83 induced root within 3.83 days in shoots cultured on media supplemented with 1.0 mg/l IBA and 0.5 mg/l NAA, whereas the variety Belela shoots have induced roots within 3.83 days on media supplemented with 1.0 mg/l IBA and 1.0 mg/l NAA. The maximum number of roots per shoot was recorded on MS media supplemented with a combination of 1.0 mg/l IBA with 0.5 mg/l NAA (11.7) followed by (9.3) on media with 0.75 mg/l IBA with 0.5 mg/l NAA, respectively. Maximum root length was observed for Beletech (3.4 cm) followed by Awassa-83 (3.43 cm) cultured on the media containing a combination of IBA 0.75 mg/l and NAA 0.5 mg/l. Acclimatization with a survival rate was 90% for Beletech and 80% for Awassa and 83% for Belela varieties | Meristem culture | [53] |
| 19. Brassica carinata A. Br. var. Holeta & yellow dodola | Micropropagation | The explant from cotyledon was more responsive (95% callusing frequency) as compared to hypocotyl (80.7%) on MS with 0.5 mg/l 2,4-D using yellow dodola. Yellow dododa gave maximum shooting (98%) in MS supplemented with 2 mg/l BAP. The highest rooting percent from a yellow dodola variety was at IBA 0.3 mg/l | callus culture (hypocotyl and cotyledon) | [29] |
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