Journals
Publish with us
Publishing partnerships
About us
Blog
Advances in Agriculture
Journal overview
For authors
For reviewers
For editors
Table of Contents
Special Issues
Advances in Agriculture
/
2024
/
Article
/
Tab 1
/
Research Article
Characterisation of the Endophytic and Rhizospheric
Bacillus licheniformis
Strains Isolated from Sweet Potato with Plant Growth-Promoting and Yield Enhancing Potential
Table 1
Primers and temperature parameters used for polymerase chain reactions.
Primers (5′−3′)
PCR parameters
References
16S rRNA gene
[
23
]
Eub 341
-F
95°C, 2 min (1 cycle)
CCTACGGGAGGCAGCAG
95°C, 30 s; 57°C, 45 s
Eub 1060
-R
72°C, 1 min (32 cycles)
CGACACGAGCTGACGACA
72°C, 7 min (1 cycle)
DNA gyrase alpha subunit gene
[
22
]
gyrA
-F
95°C, 5 min (1 cycle)
CAGTCAGGAAATGCGTACGTCCTT
94°C, 30 s; 50°C, 45 s
gyrA
-R
94°C, 30 s (30 cycles)
CAAGGTAATGCTCCAGGCATTGCT
72°C, 2 min (1 cycle)
The DNA sequences were submitted to NCBI GenBank (accession numbers OP620082-88).