Applied Bionics and Biomechanics

Applied Bionics and Biomechanics / 2016 / Article
Special Issue

Recent Advances in Biomedical Applications

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Research Article | Open Access

Volume 2016 |Article ID 7149182 | 9 pages | https://doi.org/10.1155/2016/7149182

Design Concepts of Polycarbonate-Based Intervertebral Lumbar Cages: Finite Element Analysis and Compression Testing

Academic Editor: Tadeusz Mikołajczyk
Received16 Nov 2015
Revised29 Mar 2016
Accepted18 Apr 2016
Published08 Aug 2016

Abstract

This work explores the viability of 3D printed intervertebral lumbar cages based on biocompatible polycarbonate (PC-ISO® material). Several design concepts are proposed for the generation of patient-specific intervertebral lumbar cages. The 3D printed material achieved compressive yield strength of 55 MPa under a specific combination of manufacturing parameters. The literature recommends a reference load of 4,000 N for design of intervertebral lumbar cages. Under compression testing conditions, the proposed design concepts withstand between 7,500 and 10,000 N of load before showing yielding. Although some stress concentration regions were found during analysis, the overall viability of the proposed design concepts was validated.

1. Introduction

The combination of biotechnology and 3D printing has led to the rise of 3D bioprinting, which is a processing technique that promises to solve critical issues while finding printable biomaterials, increasing the capacity of precise positioning and including cell sources, in order to be successfully applied in diagnosis, personalized medicine, and regenerative medicine [1]. Literature that demonstrates the disruptiveness of this group of applications is spread out in several fields. Radenkovic et al. suggested the idea of manufacturing personalized human hollow organs with lower architectural complexity using detailed patient information, acquired by medical imaging, appropriate cell type, and 3D printing technology [2]. Other works showed the potential of medical and industrial applications of several classes of 3D printing techniques that may be useful for attending the future demand for organ transplants. For example, Yoo made a comparison among different 3D bioprinting technologies in order to evaluate their impact on human health and medical devices industry [3]. Visser et al. stated that the potential application of 3D bioprinting in medicine will evolve into tissue printing in the near future [4].

3D printing of implants, prosthesis, and other medical devices can be considered an important stage of the full development of 3D bioprinting applications. Particularly, design of prosthesis and implants is nowadays embracing the use of 3D printing technologies as FDM (Fused Deposition Modeling), in order to solve the need for customization and the need for providing a fast response in surgical interventions [5]. Some previous works show that it is possible to satisfy the main features required in customized medical devices such as strength, sterility, dimensional stability, and safety. For example, Rankin et al. used a FDM machine for surgical retractors prototyping. This prototype was sterilized and tolerated the tangential force needed to fulfill the requirements before failure, both before and after exposure to sterilization [6].

A specific case of the need of customized implants is column surgery. This is performed in order to ease pathologies associated with back pain that are sometimes caused by deterioration of surrounding fibrous ring of intervertebral discs, resulting in spinal disc herniation. The column surgery that deals with this illness is usually known as spinal fusion surgery, where the vertebrae gradually fuse into a single body with the introduction of an intervertebral cage implant. Spinal fusion is done most commonly in the lumbar region of the spine, but it is also used to treat cervical and thoracic regions. The main function of an intervertebral cage implant is to fill the intervertebral space in order to facilitate the process of osseointegration and to provide mechanical support through an optimal load distribution and an interbody fusion balance fixation.

Figure 1 shows the conceptual representation of an intervertebral cage implant system. Typically, intervertebral cage implants are manufactured with medical grade titanium alloys (Ti6Al4V), polyether ether ketone (PEEK), and composite materials. But now, with the appearance of 3D printing and Additive Manufacturing Technologies like Fusion Deposition Modeling (FDM), the potential for developing customer oriented and reliable cage implants is higher. The following sections of this work explain the development of a process chain for taking advantage of FDM in column surgery implants customization.

There are a limited number of research studies that explore the design and rapid manufacturing of patient-specific implants for column surgery. de Beer and van der Merwe developed a study of the rapid manufacturing of metallic implants. They used computed tomography (CT), direct laser metal sintering (DMLS), and mechanical testing in order to propose a process chain for customization of intervertebral disc implants [7]. In the same matter, Domanski et al. used three Additive Manufacturing Technologies: Fused Deposition Modeling, Powder Based 3D Printing (3DP), and Selective Laser Melting (SLM). They performed Finite Element Analysis for strength validation and they compared surface roughness of each prototype developed [8]. Espalin et al. suggested the use of FDM for the freeform manufacturing of several types of implants. They concentrated their research approach on cranial reconstruction, but they suggested this technique for the customization of orthopedic spacers [9]. Chougule et al. used noninvasive imaging, reverse engineering, and FDM for the development of specific implants for minimum invasive spine surgeries. They suggested that this process chain could provide valuable medical information and powerful diagnostic tool for surgeons to understand the complex internal anatomy of the patient [10]. A work that precedes the one presented here explores the use of FDM and common 3D printing machinery, in the development of design concepts of polymeric spinal surgery implants [11]. It was an exploratory study that evaluated the feasibility of implant customization with this kind of techniques and lays the foundations of the present work.

3. Objective

There is a clear need to evaluate the viability of the use of 3D printing for the customization of column surgery implants. Thus, the main objective of this research is to validate a series of design concepts of a specific case of column implants (called “intervertebral lumbar cages”) with the aid of FDM, computational simulations, and experimental testing. Furthermore, this work is intended to lay the basis for the implementation of these medical devices in orthopedic surgeries.

4. Materials and Methods

This section covers the set-up of a proposed process chain for lumbar cage material selection, design, prototyping through FDM, mechanical testing, and Finite Element Analysis.

4.1. Materials

The proposed polymer for this research is PC-ISO (polycarbonate-ISO), an industrial thermoplastic that can be sterilized by several methods like ethylene oxide and gamma radiation, according to the work of Perez et al. In this work sterility testing was performed with successful results for material deposited with FDM process [12]. Cunha et al. evaluated safety and radiation attenuation properties of PC-ISO material and established a method for its clinical use in customized Gynecological Brachytherapy Applicators. The work concluded that PC-ISO is a suitable material for this application in a clinical setting [13].

In the case of the material biocompatibility, there are several studies ordered by the material supplier that confirm that the material is not toxic, does not present allergenic potential, and does not have irritant effects. Among the studies mentioned above there is, for example, the ISO Acute Systemic Injection Test, which was designed for screening PC-ISO extracts for potential toxic effects as a result of a single-dose systemic injection in mice. The study confirmed that the animals did not present signs of toxicity in comparison with the control. Regarding mechanical properties of the chosen material, it has a specified ultimate tensile strength of 57 MPa and a modulus of elasticity of 2 GPa, properties that made this polymer competitive with other engineered materials for implants [11].

4.2. Prototypes

The implant design for FDM took into account several design guidelines for the appropriate deposition of the polymeric material, in order to improve its mechanical performance during tensile or compressive testing and for increasing the dimensional and geometrical accuracy. Some of these design guidelines were formulated by Ahn et al., based on the results of extensive experimentation [14]. Derived from that work, the following guidelines are used in this particular case study:(i)A negative air gap, meaning that two FDM layers partially occupy the same space, will increase both strength and stiffness.(ii)The build orientation could improve the part accuracy and strength.

Prior to the exploration of patient-specific lumbar cage process chain, cylindrical specimens with two different configurations of build directions were subject to compression strength testing (Figure 2). This procedure was carried out as screening experimentation in order to establish the material mechanical properties for a Finite Element Analysis of the lumbar cage concepts. For the manufacture of the cylindrical specimens and implant design concepts, a FDM machine Stratasys Fortus 400mc was used and two different machine nozzles were configured for the deposition process (Tables 1 and 2). According to the supplier of PC-ISO material, the specific gravity is 1.20, which is equivalent to 0.0012 g/mm3 in density [15]. Taking as reference this value and the final weights of the cylindrical specimens, the fill percentage obtained with 0.30 mm nozzle is approximately 88% and the fill percentage obtained with the 0.40 mm nozzle is approximately 92%, which may influence the strength behavior.


Configuration

Build envelope ()406 × 355 × 406 mm

Material deliveryTwo build material canisters 1508 cc
Two support material canisters 1508 cc
Autochangeover between canisters

Material optionsPC-ISO

Layer thickness0.330 mm
0.254 mm
0.178 mm

Support structure Breakaway Support System (BASS™)


Nozzle diameter0.30 mm0.40 mm

Slice height 0.17 mm 0.25 mm
Contour width0.35 mm 0.50 mm
Part raster width0.35 mm 0.50 mm
Visible surface raster0.30 mm 0.43 mm
Internal raster0.40 mm 0.61 mm

The design criteria for lumbar cage geometries were established by the analysis of patents and commercial products, in which the predominant factor found was the load distribution for providing minimum damage to vertebrae [1619].

It is important to point out that the mechanical response to fatigue testing must be considered in further research since there is evidence in the literature that repetitive loading can cause vulnerability to column mechanical damage [20], and there is limited fatigue data in the literature for FDM deposited materials [21].

The evolution of all design attempts is shown in Figure 3, in which the geometries presented in previous research are also exhibited [11].

In this work, four different lumbar cage design concepts are proposed, as an optimization alternative in the search for appropriate 3D printed implants. These lumbar cage design concepts were tested in a similar set-up to that used for cylindrical specimens. Solid models of the proposed lumbar cages were drawn using a generic CAD tool. The dimensions ranges of the prototypes were about 13.4 mm in width and 28.7 mm in length, having a height of 13 millimeters. Also, all the designs included geometrical features that facilitate osseointegration. It is important to point out that the design concepts include antiskid systems required for appropriate implant fixation, but these geometrical features could generate stress concentration. Figure 4 presents the proposed lumbar cage design: (a) a flat lumbar cage that does not have an antiskid system; (b) a lumbar cage with an antiskid system with undulating features; (c) a lumbar cage with antiskid system with triangular features; and (d) the same geometry that proposed lumbar cage design (c), but with a handling hole in the side view (the handling hole is used to hold the lumbar cage during implantation).

4.3. Compression Strength Testing

Compressive strength tests for each cylindrical specimen and each lumbar cage design concept were conducted using a Shimadzu Universal Testing Machine equipped with a 25 kN load cell. The tests performed on cylindrical specimens were based on the “ASTM.D695.2010, Standard Test Method for Compressive Properties of Rigid Plastics” [22], using 6 mm/min as crosshead speed (Figure 5).

4.4. Simulation Settings

The Finite Element Analysis (FEA) was performed with the aid of COMSOL Multiphysics 4.3 software, to automatically generate tetrahedral elements and for performing the computational simulation. It is a common practice to use automatic tetrahedral mesh generators to discretize complex 3D structural components. This type of mesh generators can handle complex geometries with a minimum of human intervention (as compared to, e.g., the manual generation of a mesh of hexahedral elements). The solver used to perform the 3D calculations was embedded in the software. Mesh size was predefined in a range of 0.286 and 2.290 mm with a maximum element growth rate of 1.45. Mechanical properties of the proposed lumbar cages were assumed to be homogeneous and linear elastic. The mechanical properties of Table 3 were taken as simulation references for each proposed prototype.


Mechanical propertiesUnit0.30 mm0.40 mm

Compressive yield strengthMPa4055
Young’s modulusGPa1.22201.2580
Poisson’s ratio10.39280.4287
DensityKg/m31,0601,110

4.5. Reference Column Load

In order to establish a good approximation of the load that must be used for simulating average column loads, an additional literature review was made. Wilke et al. estimated an interdiscal pressure of 1.8 MPa, equivalent of 3,240 N in a disc area of 1800 mm2, in the L4-L5 disc while an average person was holding a 20 kg object 600 mm away from the chest [23]. Schultz et al. estimated that the interdiscal pressure varies depending on the body position and type of activity, taking values from 0.270 MPa (486 N of load over 1800 mm2) in a relaxed standing position to 1.62 MPa (2,916 N of load over 1800 mm2) in the most strenuous task examined [24]. Therefore, considering the average loads from the literature, a 4,000 N axial force was taken as reference load and the bottom face of the prototype was rigidly fixed for motion constraint.

5. Results and Discussion

5.1. Compression Strength Testing

The results of this screening experimentation for horizontal-axial build configuration were discarded since they presented lower strength behavior (7,800 N) in comparison to those with transverse-vertical build configuration for similar conditions (8,300 N). Figure 6 shows results of the compressive strength test of PC-ISO specimens manufactured by 0.30 mm nozzle in transverse-vertical build direction and 0.40 mm nozzle in transverse-vertical build direction. In order to evaluate the repeatability of the FDM process, several replications were conducted. The average of these loads was of 7,776 N and the standard deviation was of 102.3 N. Therefore, the process has a deviation error of 1.32% between the five experimental trials, a number that indicates a good repeatability of the process.

Table 3 shows the mechanical properties of PC-ISO cylindrical test specimens obtained from the initial mechanical compressive testing, in order to be used as preliminary data for the Finite Element Analysis. Compressive yield strength was calculated by dividing the load carried by the specimen at the yield point by the original minimum cross-sectional area of the specimen (126.7 mm2). Figure 6 shows the comparison of the compression test of PC-ISO cylindrical test specimens of both 0.30 mm and 0.40 mm nozzle configurations.

5.2. Simulation Results for Lumbar Cage Concepts

Simulations results are explained in terms of the average von Mises stresses exhibited in different cross sections of the four design concepts. Figure 7 shows the Finite Element Analysis of prototypes manufactured with the 0.40 mm nozzle in a full isometric view, with a top cross-sectional area of 257 mm2. Using the 4,000 N force as reference load, it is shown that the average von Mises stresses are up to 30 MPa for all prototypes. Complex geometry shows higher maximum von Mises stress. Figure 8 shows the middle cross section with an area of 268 mm2 for concepts (a), (b), and (c). Concept (d) has a middle cross section area of 262 mm2. As expected, all design concepts exhibited average von Mises stress of approximately 15 MPa. Stress increases in the outer regions of the cross section due to the complex geometry of the outside surface of the implant. In the case of concept (d), the maximum stress is higher compared to the other concepts due to the stress concentration effect of the handling hole.

Figure 9 shows the cross section of the top plane where the volumetric analysis (from Figure 7) shows the maximum von Mises stress. In this plane, the cross-sectional area is 247 mm2 due to the blind holes incorporated in the design concept. Average von Misses stress should be 16.2 MPa. Stress concentration regions produce localized values up to 78.8 MPa.

5.3. Experimental Results for Lumbar Cage Concepts

Figure 10 shows a comparison between compressive strength tests of the proposed lumbar cage design (a) manufactured by the 0.30 mm nozzle and 0.40 mm nozzle. It is clearly seen from the same figure that the lumbar cage design manufactured by the 0.40 mm nozzle has better mechanical properties than those manufactured by the 0.30 mm nozzle. Further experimentation was limited to the 0.40 mm nozzle configuration.

Figure 11 shows the measured load (L) versus displacement (d) behavior of prototypes manufactured with the 0.40 mm nozzle. The best mechanical behavior is found in those design concepts with simpler geometrical elements, such as design (a) with the flat surface and design (b) with undulating features. However, the design concepts with more complex geometry (such as (c) and (d)) facilitate osseointegration and the surgical procedure during implantation.

5.4. Discussion

Based on the cylindrical samples, the reference compressive yield strength to consider is 55 MPa. The middle plane simulation analysis shows that, on average, stress level is well below the compressive yield strength of the FDM-printed PC-ISO material. Only in the case of concept (d), the maximum stress level exceeds the limit by small percentage (6%).

From the compression testing experimental results of the cylindrical specimens and the design concepts (Figures 6 and 10), the mechanical behavior exhibited at 1 mm of displacement is similar in terms of engineering stress. Specifically, the cylindrical specimens exhibited an approximate engineering stress of 54 Mpa (estimated with a cross section of 126 mm2 and 6800 N) while the engineering stress at the middle cross section (estimated with 268 mm2 and 15,000 N) of the design concepts is approximately 55 Mpa.

Local maximum stress levels are above the material strength in some regions. For the top plane analysis, all regions with maximum stress are above the material strength (between 14 and 43% higher stress compared to the material strength). The volumetric analysis shows regions that exceed the material strength by a large percentage (up to 143% in the case of design concept (d)).

In contrast to the FEM analysis results, using 4,000 N as the reference load, the actual compression test shows that the various design concepts are robust. For the case of design concepts (a) and (b), the yield behavior starts at approximately 10,000 N. A load of approximately 7,500 N is supported by design concepts (c) and (d) before yielding is observed.

The FDM process cannot produce sharp corners due to geometry of the filament used for generation of each layer. Therefore, this additive manufacturing process is beneficial to avoid stress concentration regions in the printed intervertebral lumbar cages. In addition, the FEM analysis shows that the stress concentration areas are quite localized. Therefore, it can be concluded that the high loads supported by the different design concepts are due to a combination of (a) localized stress concentration regions, (b) additive manufacturing process that intrinsically reduces stress concentration geometries, and (c) ductile nature of the PC-ISO material.

In order to further validate the proposed design concepts, additional testing under dynamic conditions is needed in order to assess the fatigue response of the material. In terms of the design concepts, additional refinements are required in order to reduce the amount of stress concentration due to sharp changes in geometry.

6. Conclusions and Future Work

This work has shown the viability of FDM-printed intervertebral lumbar cages based on biocompatible polycarbonate (PC-ISO material). Several design concepts are proposed for the generation of patient-specific intervertebral lumbar cages. Finite Element Analysis and compression testing show the viability of the proposed design concepts. The part interior style has a more significant influence than the build direction of the material deposition. Furthermore, PC-ISO material showed a high repeatability in the manufacture process for transverse-vertical build direction and solid part interior style parameters, achieving compressive yield strength of 55 MPa. The literature recommends a reference load of 4,000 N for design of intervertebral lumbar cages. Under compression testing conditions, the FDM-printed intervertebral lumbar cages withstand between 7,500 and 10,000 N of load before showing yielding.

Further research must be carried out with in vitro and in vivo testing in order to guarantee the full viability of the intervertebral lumbar cage implants that have been proposed in this work. Specifically, the validation should be carried out through the analysis of biocompatibility and osseointegration with the surrounding tissue, towards the full integration of 3D printed implants in the medical practice. The benefits of using 3D printing for each specific patient should potentially increase the ergonomics, simplify the procedure, and bring overall better personalized results once validated.

Competing Interests

The authors declare that there are no competing interests regarding the publication of this paper.

Acknowledgments

The authors would like to acknowledge the support of Tecnológico de Monterrey through its Research Group in Advanced Manufacturing. Support was also provided from the MADiT “National Lab of Additive Manufacturing, 3D Digitizing and Computerized Tomography” at Universidad Nacional Autónoma de México and from Centro de Tecnologia da Informação Renato Archer. Finally, the authors of this work would like to express their gratitude to Jan Lammel Lindemann, for his valuable comments.

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Copyright © 2016 J. Obedt Figueroa-Cavazos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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