Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon Stem/Progenitor Cells

Figure 4

Cell viability on days 1, 2, and 3 was detected using the CCK-8 assay in different groups (a). Cytoskeleton and nucleus staining of TSPCs on different SF film groups and control group and also the distribution of normal tendon fibers and tendon cells (b). Quantitative analysis of the ratio of oriented aligned cells, cell body aspect, major axis angle, and cell area of TSPCs in different groups (c). Comparison of the expression levels of tendon-related genes after 3 days and 7 days (d). Group C was the normal culture plate group, group S was the SF film group with a smooth surface, and the other groups were SF film groups with different microstructure sizes (5, 10, 15 and 20 μm). indicates , indicates .

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