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Analytical Cellular Pathology
Volume 16, Issue 3, Pages 125-130

Detection of Human Papillomavirus Type 2 Related Sequence in Oral Papilloma

Taihei Yamaguchi,1,2,5 Masanobu Shindoh,3,5 Akira Amemiya,3 Nobuo Inoue,4 Masaaki Kawamura,4 Hiroshi Sakaoka,2 Masakazu Inoue,1 and Kei Fujinaga5

1Department of Preventive Dentistry, Kagoshima University Dental School, Kagoshima, Japan
2Department of Oral Microbiology, Hokkaido University Dental School, Sapporo, Japan
3Department of Oral Pathology, Hokkaido University Dental School, Sapporo, Japan
4Department of Oral Surgery, Hokkaido University Dental School, Sapporo, Japan
5Department of Molecular Biology, Sapporo Medical College, Sapporo, Japan

Received 2 June 1997; Accepted 26 February 1998

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Oral papilloma is a benign tumourous lesion. Part of this lesion is associated with human papillomavirus (HPV) infection. We analysed the genetical and histopathological evidence for HPV type 2 infection in three oral papillomas. Southern blot hybridization showed HPV 2a sequence in one lesion. Cells of the positive specimen appeared to contain high copy numbers of the viral DNA in an episomal state. In situ staining demonstrated virus capsid antigen in koilocytotic cells and surrounding cells in the hyperplastic epithelial layer. Two other specimens contained no HPV sequences by labeled probe of full length linear HPVs 2a, 6b, 11, 16, 18, 31 and 33 DNA under low stringency hybridization conditions. These results showed the possibility that HPV 2 plays a role in oral papilloma.