Table of Contents Author Guidelines Submit a Manuscript
Analytical Cellular Pathology
Volume 23, Issue 2, Pages 61-64
http://dx.doi.org/10.1155/2001/301570

High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1

Jacob Larsen,1 Anne Marie Ottesen,2 Maria Kirchhoff,3 Claes Lundsteen,3 and Jørgen K. Larsen1

1Finsen Laboratory, Finsen Center, Rigshospitalet, Strandboulevarden 49, DK‐2100, Copenhagen, Denmark
2Department of Growth and Reproduction, Juliane Marie Center, Rigshospitalet, Blegdamsvej 9, DK‐2100, Copenhagen, Denmark
3Department of Clinical Genetics, Juliane Marie Center, Rigshospitalet, Blegdamsvej 9, DK‐2100, Copenhagen, Denmark

Received 12 March 2001; Accepted 8 October 2001

Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) as opposed to the use of unamplified DNA. Five DNA samples from B‐cell leukemias with small 11q deletions were amplified by DOP‐PCR and analysed by means of high resolution comparative genomic hybridization (HR‐CGH) for the evaluation of aberration size detection limit. By means of HR‐CGH, we found the detection limit of DOP‐PCR CGH for deletions to be between 3 Mbp and 7–8 Mbp.