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Analytical Cellular Pathology
Volume 33 (2010), Issue 3-4, Pages 113-119
http://dx.doi.org/10.3233/ACP-CLO-2010-0544

Gene Copy Number Variation in Male Breast Cancer by aCGH

Stefania Tommasi,1 Anita Mangia,1 Giuseppina Iannelli,1 Patrizia Chiarappa,1 Elena Rossi,2 Laura Ottini,3 Marcella Mottolese,4 Wainer Zoli,5 Orsetta Zuffardi,2 and Angelo Paradiso1

1Clinical Experimental Oncology Laboratory, National Cancer Centre of Bari, Bari, Italy
2Department of General Biology and Medical Genetics, University of Pavia, Pavia, Italy
3Department of Molecular Medicine, University La Sapienza, Rome, Italy
4Division of Medical Oncology A, Regina Elena Cancer Institute, Rome, Italy
5Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori, Meldola (FC), Italy

Copyright © 2010 Hindawi Publishing Corporation and the authors. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background: Male breast cancer (MBC) is a rare disease and little is known about its etiopathogenesis. Array comparative genomic hybridization (aCGH) provides a method to quantitatively measure the changes of DNA copy number and to map them directly onto the complete linear genome sequences. The aim of this study was to investigate DNA imbalances by aCGH and compare them with a female breast cancer dataset.

Methods: We used Agilent Human Genome CGH Microarray Kit 44B and 44K to compare genomic alterations in 25 male breast cancer tissues studied at NCC of Bari and 16 female breast cancer deposited with the Gene Expression Omnibus (GSE12659). Data analysis was performed with Nexus Copy Number 5.0 software.

Results: All the 25 male and 16 female breast cancer samples displayed some chromosomal instability (110.93 alterations per patient in female, 69 in male). However, male samples presented a lower frequency of genetic alterations both in terms of loss and gains.

Conclusion: aCGH is an effective tool for analysis of cytogenetic aberrations in MBC, which involves different biological processes than female. Male most significant altered regions contained genes involved in cell communication, cell division and immunological response, while female cell–cell junction maintenance, regulation of transcription and neuron development.