Analytical Cellular Pathology

Analytical Cellular Pathology / 2010 / Article

Open Access

Volume 33 |Article ID 917013 | 15 pages | https://doi.org/10.3233/ACP-CLO-2010-0538

Dual Effects of β3 Integrin Subunit Expression on Human Pancreatic Cancer Models

Abstract

Background: Pancreatic cancer, the fifth leading cause of adult cancer death in Western countries, lacks early detection, and displays significant dissemination ability. Accumulating evidence shows that integrin-mediated cell attachment to the extracellular matrix induces phenotypes and signaling pathways that regulate tumor cell growth and migration.Methods: In view of these findings, we examined the role of β3 in pancreatic cancer by generating two stable β3-expressing pancreatic human cell lines and characterizing their behavior in vitro and in vivo.Results: Transduction of β3 selectively augmented the functional membrane αvβ3 integrin levels, as evident from the enhanced adhesion and migration abilities related to active Rho GTPases. No effects on in vitro anchorage-dependent growth, but higher anoikis were detected in β3-overexpressing cells. Moreover, tumors expressing β3 displayed reduced growth. Interestingly, treatment of mice with an αv-blocking antibody inhibited the growth of β3-expressing tumors to a higher extent.Conclusion: Our results collectively support the hypothesis that αvβ3 integrin has dual actions depending on the cell environment, and provide additional evidence on the role of integrins in pancreatic cancer, which should eventually aid in improving prediction of the effects of therapies addressed to modulate integrin activities in these tumors.

Copyright © 2010 Hindawi Publishing Corporation and the authors. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


More related articles

334 Views | 286 Downloads | 4 Citations
 PDF  Download Citation  Citation
 Order printed copiesOrder

Related articles

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.