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Analytical Cellular Pathology
Volume 36 (2013), Issue 3-4, Pages 63-69
http://dx.doi.org/10.3233/ACP-130081

Urothelial Differentiation of Human Umbilical Cord-Derived Mesenchymal Stromal Cells In Vitro

Shuai Wu,1,2,3 Zhongliang Cheng,1,2 Guohua Liu,2 Xinfeng Zhao,4 Liang Zhong,2 Yingjian Zhu,2 and Jiang Zhu2

1School of Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, China
2Department of Urology, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, China
3Department of Urology, Qingdao Municipal Hospital, Qingdao, China
4Section of Animal Models for Human Disease, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, China

Copyright © 2013 Hindawi Publishing Corporation and the authors. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Human umbilical cord-derived mesenchymal stromal cells (hUCMSCs) are the most primitive of those isolated from other post-natal tissue source. The hUCMSCs possess the capability of differentiating along multi-lineage. This study aimed to investigate whether hUCMSCs can differentiate into urothelium-like cells. The hUCMSCs were isolated from fresh human umbilical cord postpartum and expanded at least to passage 3 in vitro. Subsequently, they were cultured with conditioned medium from urothelial cells (UC-CM) supplemented with 20 ng/ml exogenous epidermal growth factor (EGF). Urothelial cell specific marker uroplakin II (UPII) and cytokeratins were evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence technology. During culture, hUCMSCs started to express UPII and cytokeratins weakly at 7 days and were significantly up-regulated at 2 weeks post-induction. Additionally, morphology of hUCMSCs changed from spindle-shape to a polygonal epithelial-shape similar to that of urothelial cells after 7 days. The study results indicated that hUCMSCs can differentiate into urothelium-like cells in a defined micro-environment in vitro constituted by UC-CM and exogenous EGF.