Prognostic Efficacy of Nuclear Morphometry at Invasive Front of Oral Squamous Cell Carcinoma: An Image Analysis Microscopic Study

Figure 6

This pictorial diagram represents the stepwise procedure of image analysis after standardization. Subtract picture (1) from (2) to obtain a subtracted image (3). Subtracted image is converted to a grey level image (4) in step 2. Set the threshold (Figure (5)). Image (4) is thresholded (picture (6)). Then set the scale (picture (7)). Mark the nuclei using Wand tool (picture (8)). Set the measurements as depicted (picture (9)). Save measurements in an Excel format (picture (10)).