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Analytical Cellular Pathology
Volume 2014, Article ID 963076, 13 pages
Research Article

Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas

1Department of Physics, School of Natural Sciences, University of Patras, Rio, 26504 Patras, Greece
2Medical Image and Signal Processing Laboratory, Department of Biomedical Engineering, Technological Educational Institute of Athens, Ag. Spyridonos, Egaleo, 12210 Athens, Greece
3Department of Pathology, University Hospital of Patras, 26504 Rio, Greece
4Department of Medical Physics, School of Health Sciences, Faculty of Medicine, University of Patras, Rio, 26504 Patras, Greece

Received 4 June 2014; Accepted 16 November 2014

Copyright © 2014 Konstantinos Ninos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. P63 immunostaining has been considered as potential prognostic factor in laryngeal cancer. Considering that P63 is mainly nuclear stain, a possible correlation between the texture of P63-stained nuclei and the tumor’s grade could be of value to diagnosis, since this may be related to biologic information imprinted as texture on P63 expressed nuclei. Objective. To investigate the association between P63 stained nuclei and histologic grade in laryngeal tumor lesions. Methods. Biopsy specimens from laryngeal tumour lesions of 55 patients diagnosed with laryngeal squamous cell carcinomas were immunohistochemically (IHC) stained for P63 expression. Four images were digitized from each patient’s IHC specimens. P63 positively expressed nuclei were identified, the percentage of P63 expressed nuclei was computed, and 118 textural, morphological, shape, and architectural features were calculated from each one of the 55 laryngeal lesions. Data were split into the low grade (21 grade I lesions) and high grade (34 grade II and grade III lesions) classes for statistical analysis. Results. With advancing grade, P63 expression decreased, P63 stained nuclei appeared of lower image intensity, more inhomogeneous, of higher local contrast, contained smaller randomly distributed dissimilar structures and had irregular shape. Conclusion. P63 expressed nuclei contain important information related to histologic grade.