Fractal Analysis and the Diagnostic Usefulness of Silver Staining Nucleolar Organizer Regions in Prostate Adenocarcinoma

Figure 3

Example of binarisation and pruning filtering. After segmentation of original images ((a), (d)), argyrophilic nucleolar organizer regions (AgNORs) are binarised ((b), (e)) and then skeletons of the AgNOR dots are extracted ((c), (f)) in order to offer a quantifiable view of the glandular distribution of the dots. Scale bar represents 100 μm.

(a)

(b)

(c)

(d)

(e)

(f)