Comparison of the Manual, Semiautomatic, and Automatic Selection and Leveling of Hot Spots in Whole Slide Images for Ki-67 Quantification in Meningiomas

Figure 5

The results of hot spot and area of quantification selections by one pathologist ((a), (b), and (c)) and by proposed automated method (d) with examples of 3 areas of quantification chosen approximately in the same region (hot spot) for each selection. The Ki-67 index value calculated as percentage of immunopositive nuclei to the whole number of nuclei in presented area differs slightly.

(a)

(b)

(c)

(d)