Analytical Cellular Pathology / 2015 / Article / Fig 1

Research Article

Ex Vivo Nicotine Stimulation Augments the Efficacy of Human Peripheral Blood Mononuclear Cell-Derived Dendritic Cell Vaccination via Activating Akt-S6 Pathway

Figure 1

Nicotine upregulates surface molecules expression in human semimature DCs. (a–d) DCs derived from human PBMC with 100 ng/mL recombinant human GM-CSF and IL-4 were conferred with α-bungarotoxin (2 μg/mL) or tubocurarine chloride (4 × 10−5 mol/L) 2 h prior to nicotine (10−7 mol/L) 12~15 h stimulation. The effect of nicotine on the expression of CD80 (a), 4-1BBL (b), MHC class I (c), and MHC class II (d) molecules was determined by flow cytometry. Numbers in histogram indicated mean fluorescence intensity (MFI) of test samples. Data were given as mean ± SEM, one-way ANOVA with post-Newman-Keuls test. , , and . One representative from 3 independent experiments is shown. Ni: nicotine; BTX: α-bungarotoxin; and TC: tubocurarine chloride.
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