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Analytical Cellular Pathology
Volume 2015 (2015), Article ID 920409, 8 pages
http://dx.doi.org/10.1155/2015/920409
Research Article

Intratumoral CD3+ T-Lymphocytes Immunoexpression and Its Association with c-Kit, Angiogenesis, and Overall Survival in Malignant Canine Mammary Tumors

1CECAV, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
2Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
3School of Life Sciences and the Environment, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
4Centro Hospitalar Veterinário, Rua Manuel Pinto de Azevedo 118, 4100-320 Porto, Portugal
5Hospital Veterinário do Porto, Travessa Silva Porto 174, 4250-475 Porto, Portugal
6Faculdade de Medicina Veterinária, Universidade Lusófona de Humanidades e Tecnologias, Campo Grande 376, 1749-024 Lisboa, Portugal
7Center for the Study of Animal Sciences, CECA-ICETA, University of Porto, 4051-401 Porto, Portugal
8Center for Research and Technology of Agro-Environment and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal

Received 28 April 2015; Accepted 9 July 2015

Academic Editor: Giovanni Tuccari

Copyright © 2015 Maria Isabel Carvalho et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this study 80 malignant CMT were submitted to immunohistochemical detection of CD3, c-kit, VEGF, and CD31, together with clinicopathological parameters of tumor aggressiveness. CD3+ T-cells and c-kit overexpression revealed a positive correlation with VEGF (r = 0.503, P < 0.0001; r = 0.284, P = 0.023 for CD3 and c-kit, resp.) and CD31 (r = 0.654, P < 0.0001; r = 0.365, P = 0.003 for CD3 and c-kit, resp.). A significant association (P = 0.039) and a positive correlation (r = 0.263, P = 0.039) between CD3 and c-kit were also observed. High CD3/VEGF, c-kit/VEGF, and CD3/c-kit tumors were associated with elevated grade of malignancy (P < 0.0001 for all groups), presence of intravascular emboli (P < 0.0001 for CD3/VEGF and CD3/c-kit; P = 0.002 for c-kit/VEGF), and presence of lymph node metastasis (P < 0.0001 for all groups). Tumors with high CD3/VEGF (P = 0.006), c-kit/VEGF (P < 0.0001), and CD3/c-kit (P = 0.002) were associated with poor prognosis. Interestingly high c-kit/VEGF tumors retained their significance by multivariate analysis arising as independent prognostic factor.