MMP-2 and MMP-9 Activities and TIMP-1 and TIMP-2 Expression in the Prostatic Tissue of Two Ethanol-Preferring Rat Models

Figure 1

Representative gelatin-zymography of dorsal prostate (DP) and lateral prostate (LP) from low (UChA) and high (UChB) ethanol-preferring rats. UChAC and UChBC are respective controls. Clear bands of gelatinolytic activity for MMP-2 (64 kDa, intermediate form) and MMP-9 (81 kDa, active form) were observed. Lane S corresponds to the reference standard of human pro-MMP9 and active-MMP2 enzymes. Ethanol intake slightly reduces the MMP-2 activity in the dorsal lobe of both rat models and in the lateral lobe of UChB rats but significantly reduces it in the lateral lobe of UChA rats. MMP-9 activity was slightly reduced in dorsal lobe but was significantly reduced in the lateral lobe of both rat models, mainly in the UChB rats. The histograms represent the values of densitometric analysis of the bands. Data are expressed as the mean ± SD (; ; ).

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