In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes

Figure 5

CTC size analysis using two-color two-beam in vivo PAFFC platform. (a) Time-of-flight data for cells after injection in a slightly constricted artery. Inset shows linear cells velocity measured from time delay () between signals. (b) Typical fluorescence signals from slow (left) and fast (right) cells. Arrows indicate FWHM and delay parameters acquired for each cell. (c) Cell (and cell aggregates) size calculated from time-of-flight and cell velocity data. (d) Cell (and cell aggregates) size distribution histogram calculated from in vivo data.

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