Research Article

Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas

Table 2

Automatic method used to determine label index with basic and advanced ImmunoRatio. The table shows the total of tumorous cells obtained from ameloblastoma by the use of techniques of ImmunoRatio; the kappa index obtained for each range is also shown. The kappa index ranged from substantial to almost perfect for all the advanced methods of ImmunoRatio in all cases. κ = (scoring 1 versus 2) and . For the basic methods of ImmunoRatio, the Kappa index was moderate κ = (scoring 1 versus 2) and without statistical significance for the UA. The advanced methods: IRA scoring (ImmunoRatio advanced scoring) and IRpA scoring (ImmunoRatio plugin advanced scoring). Basic methods: IRB scoring (ImmunoRatio basic scoring), IRpB (ImmunoRatio plugin basic scoring). IRA scoring and IRB scoring were used with the webpage of ImmunoRatio. IRpA and IRpB were used with the program ImageJ and the plugin of ImmunoRatio.

AM, (100%)Total cell count ± SDIRA scoring (1) ± SDIRpA scoring (2) ± SD = (scoring 1 versus 2)

UA15, (44.1)N/A0.8560.000
SMA19, (55.8)N/A0.7260.000

AM, (100%)Total cell count ± SDIRB scoring (1) ± SDIRpB scoring (2) ± SD = (scoring 1 versus 2)

UA15, (44.1)N/A0.0540.057
SMA19, (55.8)N/A0.0500.000