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Analytical Cellular Pathology
Volume 2016, Article ID 9084909, 7 pages
http://dx.doi.org/10.1155/2016/9084909
Research Article

Image Montaging for Creating a Virtual Pathology Slide: An Innovative and Economical Tool to Obtain a Whole Slide Image

1Oral Diagnostic and Surgical Science Division, International Medical University, No. 126, Jalan 19/155B, 57000 Bukit Jalil, Kuala Lumpur, Malaysia
2Oral Pathology and Oral Medicine Division, Faculty of Dentistry, MAHSA University, Bandar Saujana Putra, 41200 Jenjarom, Selangor, Malaysia
3Restorative Dentistry Division, International Medical University, No. 126, Jalan 19/155B, 57000 Bukit Jalil, Kuala Lumpur, Malaysia
4MDS, Oral and Maxillofacial Pathology, 12-13-36, Lakshmi Nivas, Tarnaka, Hyderabad 500017, India
5Oral Pathology, JSS Dental College and Hospital, Sri Shivarathreeshwara Nagara, Bannimantap, Mysore 570015, India

Received 25 May 2016; Revised 12 July 2016; Accepted 1 September 2016

Academic Editor: Fernando Schmitt

Copyright © 2016 Spoorthi Ravi Banavar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Microscopes are omnipresent throughout the field of biological research. With microscopes one can see in detail what is going on at the cellular level in tissues. Though it is a ubiquitous tool, the limitation is that with high magnification there is a small field of view. It is often advantageous to see an entire sample at high magnification. Over the years technological advancements in optics have helped to provide solutions to this limitation of microscopes by creating the so-called dedicated “slide scanners” which can provide a “whole slide digital image.” These scanners can provide seamless, large-field-of-view, high resolution image of entire tissue section. The only disadvantage of such complete slide imaging system is its outrageous cost, thereby hindering their practical use by most laboratories, especially in developing and low resource countries. Methods. In a quest for their substitute, we tried commonly used image editing software Adobe Photoshop along with a basic image capturing device attached to a trinocular microscope to create a digital pathology slide. Results. The seamless image created using Adobe Photoshop maintained its diagnostic quality. Conclusion. With time and effort photomicrographs obtained from a basic camera-microscope set up can be combined and merged in Adobe Photoshop to create a whole slide digital image of practically usable quality at a negligible cost.