Long Noncoding RNA MIR4697HG Promotes Cell Growth and Metastasis in Human Ovarian Cancer

Figure 3

Knockdown of MIR4697HG inhibits cell viability and clonogenic potential in SKOV3 cells and OVCAR3 cells. (a) SKOV3 cells with (shRNA group) or without (NC group) specific shRNA transfections were subject to cell viability assessment for consecutive 5 days. (b) OVCAR3 cells with (shRNA group) or without (NC group) specific shRNA transfections were subject to cell viability assessment for consecutive 5 days. (c) Both SKOV3 cells and OVCAR3 cells with (shRNA group) or without (NC group) specific shRNA transfections were subject to colony formation assays. After crystal violet staining, colonies were visually less observed in MIR4697HG-depleted SKOV3 cells and OVCAR3 cells. (d) Counting of colonies showed that colony numbers were significantly decreased by 75% in MIR4697HG-depleted SKOV3 cells and 66% in MIR4697HG-depleted OVCAR3 cells. .

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