Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells

Figure 5

SSP induces polyploidy and polynucleation in A549 cells. (a, b) FACS analysis of A549 cells that had been cultivated in normal growth medium with (control) or without (−serum) 10% FCS or in growth medium with 10% FCS containing 20 or 50 nM SSP. FACS plots are shown in (a), whereas in (b), the respective relative cell numbers in representative sections of micrographs in (a) are given on the y-axis. (c) DAPI staining to reveal the nuclear morphology of A549 cells that had been cultivated in the absence or presence of 50 nM SSP for 7 or 21 days. The corresponding phase micrographs are also given. In addition, a lower magnification of 21-day treated A549 cells is shown at the right bottom of (c) to reveal a larger cell number.

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