Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells

Figure 6

SSP-induced stop of proliferation is reversible. (a) Short- and long-time cultures of A549 cells in the absence or presence of 50 nM SSP. A549 cells were cultivated at the indicated time points with or without 50 nM SSP, stained with DAPI, and the cell number in selected areas was determined microscopically. Cell number at day zero (d 0) was set as 100%, and the other values were adjusted accordingly. (b) A549 cells that had been cultured in the presence of 50 nM SSP for three weeks were cultured in the absence of SSP for further three weeks. A colony of dividing cells in a dawn of still more flattened cells is indicated with an arrow. Fluorescence (DAPI label) and corresponding phase micrographs are shown.

(a)

(b)