Research Article

Molecular Detection of EMT Markers in Circulating Tumor Cells from Metastatic Non-Small Cell Lung Cancer Patients: Potential Role in Clinical Practice

Figure 1

Schematic drawing of the immunomagnetic isolation and detection of A549EMT cells. After negative enrichment by immunomagnetic depletion of leukocytes, putative A459EMT cells spiked in the normal blood samples were analyzed with quantitative immunofluorescence microscopy, cytofluorimetry, and RT-PCR assay.