Human Cellular Models for the Investigation of Lung Inflammation and Mucus Production in Cystic Fibrosis

Figure 3

Neutrophil transmigration in AEC culture. AECs are seeded on the opposite side of filter inserts and allowed to attach, then the insert is inverted and can be cultured either submerged or at ALI (not shown). Neutrophils are added on the top chamber representing the basolateral surface of AECs and induced to migrate to the lower chamber against a chemoattractive agent (e.g., fMLP). After 3–4 hours, neutrophils are recovered from the lower chamber and percentage of migrated cells over the total is calculated.