Erythrocyte Senescence in a Model of Rat Displaying Hutchinson-Gilford Progeria Syndrome

Figure 5

Erythrocyte GSH content and L-cysteine influx values represented as mean ± SD of eight independent experiments. (a) Effect of DHT treatment on GSH content of young, old, and unfractionated erythrocytes and its comparison with values of young and old. The represented values are mean ± SD of eight experiments performed independently. Overall erythrocyte GSH content in old erythrocytes of the young control group and the old control group was significantly () lower as compared to the young and unfractionated erythrocytes of the same group. The progeria induction significantly () decreased the overall GSH content in young, old, and unfractionated erythrocytes as compared to the corresponding erythrocytes of the young control group, but the level was higher than that of the naturally aged old control group. (b) L-cysteine influx of erythrocyte measured as marker of aging and membrane damage. The values represent the mean ± SD of eight independently performed experiments. L-cysteine influx was found to decrease significantly () as function of age with lower values in different erythrocyte fractions of naturally aged old rats as compared to the corresponding young control group. The progeroid rats were associated with significant () decline in L-cysteine influx compared the age-matched young control group, but the level was higher than that of the old control group.

(a)

(b)