Tremella fuciformis Polysaccharides Attenuate Oxidative Stress and Inflammation in Macrophages through miR-155

Figure 7

TFPS attenuates LPS-induced inflammation reaction by inhibition of miR-155. (a) The miR-155 expression levels in control, LPS-treated (1 μg/ml), TFPS-treated (200 μg/ml), and TFPS-pretreated (200 μg/ml) + 1 μg/ml LPS-treated (LPS + TFPS) cells, as assessed by real-time RT-qPCR (, versus control; versus the LPS + TFPS group). (b) The miR-155 expression levels in control, TFPS-treated (200 μg/ml), LPS-treated (1 μg/ml), and 1 μg/ml LPS-treated cells following TFPS pretreatments (10, 50, 100, 200, or 300 μg/ml) of RAW264.7 cells for 1 h (, versus control; , versus the LPS group). (c) ELISAs of TNF-α and IL-6 in control, LPS + NC, LPS + miR-155, LPS + NC + TFPS, and LPS + miR-155 + TFPS groups (, , ).

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