Metformin Treatment Inhibits Motility and Invasion of Glioblastoma Cancer Cells

Figure 5

Effect of metformin on SF268 cell motility is mimicked by inhibiting PI3K. (a) Cells were treated with metformin for 24 h before blotting for Akt and p-Akt. (b) Densitometry analysis was performed using the ImageJ software, and the values were normalized to the control. Data are the mean ± SEM from 3 independent experiments. The results were significant with . (c) SF268 cells were treated with wortmannin (100 nM) (Supplemental movie S6) or with DMSO alone (Supplemental movie S5). Quantitation of their net path is expressed in μm. Data are the mean ± SEM. indicates that the values are significant with .

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