Prognostic Significance of Cyclins A2, B1, D1, and E1 and CCND1 Numerical Aberrations in Oral Squamous Cell Carcinomas

Table 3

Univariate analysis of overall survival and disease-free survival at 5 years, according to clinicopathological characteristics and marker expression.

Factor	Group	N	Overall survival (5 years)	value	N	Disease-free survival (5 years)	value
Gender	Female	15	64.2	0.206	14	50	0.442
	Male	52	47.3		50	30.7
Age	<59 years	33	44.4	0.500	33	30.6	0.534
	≥59 years	34	54.6		31	39.6
Location	Tongue	28	46.5	0.261	28	44.5	0.688
	Floor of the mouth	20	44.4		20	25
	Gingiva	7	28.6		5	40
	Retromolar trigone	4	50		3	33.3
	Hard palate	5	80		5	20
	Buccal mucosa	3	0		3	66.7
Tumour size	T1	22	71.5	<0.001	22	58.3	<0.001
	T2	27	53.2		27	30.5
	T3	6	33.3		6	22.2
	T4	12	8.3		9	0
N status	N0	46	68.2	<0.001	46	44.9	<0.001
	N1	11	9.1		10	12
	N2	10	10		8	0
Stage	I	21	74.9	<0.001	21	61.1	<0.001
	II	20	67.8		20	43.3
	III	11	27.3		11	21.2
	IV	15	6.7		12	0.0
Treatment	SG	49	53.2	0.340	48	34	0.964
	SG + RT	18	38.9		16	35.7
Tumour histological grade	G1	34	63.7	0.147	32	47.3	0.011
	G2	29	36.1		28	27.2
	G3	4	25		4	0
Margin status	Free of tumour	54	51.9	0.211	53	37.8	0.757
	With tumour	9	33.3		8	37.5
Vascular invasion	Absent	62	50.1	0.620	59	33.9	0.886
	Present	5	40		5	40
Perineural permeation	Absent	56	54	0.039	54	34.2	0.632
	Present	11	27.3		10	37.5
Cyclin A2	<25%	40	63.3	0.005	40	43.8	0.203
	≥25%	25	30.8		22	22.3
Cyclin B1	<10%	26	57	0.331	25	32	0.526
	≥10%	39	46.9		37	39.2
Cyclin D1	<50%	26	60.6	0.172	24	49.4	0.236
	≥50%	39	44.8		38	26.3
Cyclin E1	<10%	43	48.2	0.233	40	33.9	0.758
	≥10%	21	59.1		21	41.6
Ki-67	<50%	35	58.6	0.111	35	38.1	0.988
	≥50%	29	44.0		26	36.1
CCND1	Normal	34	52.3	0.991	32	40.2	0.412
	Numerical aberration	26	47.1		25	29.7

SG: surgery; RT: radiotherapy. Not available in 4 cases. The number of patients in the disease-free survival in some of the analyses is different from the overall survival analysis as some patients had persistence of the disease and were only evaluated in the overall survival.