Loss of BRCA1 Spontaneously Induces the Tumorigenesis in Lacrimal Gland

Figure 4

Overexpression of cyclin D1 and increased cell proliferation in lacrimal gland tumors from Brca1-mutant mice. (a) Protein expression patterns in lacrimal gland tumors (T1 (10 M), T2 (11 M), T3 (12 M), and T4 (14 M)) from Brca1^co/coMMTV-cre mice compared with the normal tissue (N1, N2, N3, and N4). β-Actin was used as a loading control. (b–j) Lacrimal gland sections from tumor areas of Brca1^co/coMMTV-cre mice stained with H&E (b, e, h) and immunostained for PCNA (c, f, i) and cyclin D1 (d, g, j). Yellow lines in (c) and (d) separate tumor (T; upper left) and normal (N; lower right) tissue. Scale bars, 200 μm.