LncRNA LOXL1-AS1 Promotes the Proliferation and Metastasis of Medulloblastoma by Activating the PI3K/AKT Pathway

Figure 6

Knockdown of LOXL1-AS1 inhibited cell metastasis and reversed EMT processes in medulloblastoma. (a, b) Control or shRNA-treated D283 and D341 cells were subject to wound-healing assay. Representative images showing the wound recovery were shown at 0 h and 18 h for both cell lines. The wound-recovered area which represented the cell migration capacity was calculated for each group of cells. (c, d) Both D283 and D341 cells were subject to Transwell migration assay. Cells that migrated to the lower surface were stained with crystal violet. Transmigrated cells were counted and averaged from 5 randomly selected fields. (e) Immunofluorescent analysis of vimentin (mesenchymal marker) and E-cadherin (epithelial marker). (f) Western blot analysis of vimentin and E-cadherin in D283 and D341 cells. Expression of vimentin was less detected, whilst that of E-cadherin was largely detected after knockdown of LOXL1-AS1. ; .

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