Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin

Figure 5

Analysis of cyclin D1 expression and correlation with wtKLF6 and KLF6-SV1. Total proteins extracted from nontumor (N) and tumor samples (T1–T8) were analyzed by Western blot using a monoclonal anti-cyclin D1 antibody followed by HRP-conjugated anti-rabbit IgG, and the signal was revealed by the ECL plus kit (Amersham Biosciences). Ponceau staining was represented in the upper part of (a). Association between the cyclin D1 and the expression levels of KLF6-SV1 (b) and wtKLF6 (c) in tumor tissues. Cyclin D1+ (value densitometry > 1) and cyclin D1− (value densitometry < 1).

(a)

(b)

(c)