Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin

Table 1

Primer sequences and product sizes of KLF6 (wild type, SV1, SV2, and SV3), CDH1, and GAPDH.

Gene	Primer sequence (5 to 3)	Product size (bp)	Annealing T (°C)
wtKLF6 (RT-QPCR)
Forward	CGGACGCACACAGGAGAAAA	236	61
Reverse	CTCAGCCTGGAAGCCTTTTA
KLF6-SV1
Forward	CCTCGCCAGGGAAGGAGAA	237	61
Reverse	CTCAGCCTGGAAGCCTTTTA
KLF6-SV2
Forward	TCGGGGAAGCCAGGAGAA	236	61
Reverse	CTCAGCCTGGAAGCCTTTTA
KLF6-SV3
Forward	CGGACGCACACAGGTGTT	112	61
Reverse	CTCAGCCTGGAAGCCTTTTA
KLF6-WT (CNV)
Forward	CAGTCCTCCAGAGGACACTC	174	60
Reverse	CTCAATTTTCCCGAGCTGACCA
GAPDH
Forward	ACCCACTCCTCCACCTTTG	178	60
Reverse	CTCTTGTGCTCTTGCTGGG
CDH1
Forward	ACCCACTCCTCCACCTTTG	180	60
Reverse	CTCTTGTGCTCTTGCTGGG