GTSE1, CDC20, PCNA, and MCM6 Synergistically Affect Regulations in Cell Cycle and Indicate Poor Prognosis in Liver Cancer

Figure 2

Cell proliferation of Bel-7404 cells with GTSE1 knockdown. (a) Expression level of GTSE1 mRNA using real-time quantitative PCR and Western blotting. Triple experiments were performed. GAPDH was used as the endogenous control. Values were represented as the . . (b) Western blotting of GTSE1 protein. (c) Representative images of BEL-7404 cells in control and shRNA infection from day 1 to day 5. (d, e) Cell proliferation after GTSE1 knockdown was determined by the Celigo image cytometer for 5 days. (d) Cell count curves of shGTSE1 (psc34526) and shGTSE1 (psc34528) and the control group (shCtrl) over time. (e) Curves of fold change of the cell count of shGTSE1 (psc34526) and shGTSE1 (psc34528) and the control group (shCtrl) over time. Triplicate for each group; values were represented as the . The cell proliferation rate of the control group is significantly higher than those in the shGTSE1 groups (). The differences between the control group (shCtrl) and shGTSE1 (psc34526) were analyzed by a -test. and .

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