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Figure 3: ILF2 is an upstream protein of CREB. (a, b) The ILF2, CREB, and GAPDH protein expression levels in control (infected with shRNA against GFP), Bel-7402, or SMMC-7721 cells with knockdown and overexpression of CREB or ILF2 were measured by western blotting. (c–e) Cell proliferation, colony formation capacity, and apoptosis status in control cells and Bel-7402 or SMMC-7721 cells with indicated plasmids transfected. (f–h) Cell proliferation, colony formation capacity, and apoptosis status in control cells and Bel-7402 or SMMC-7721 cells with indicated plasmids transfected. Data were exhibited as mean ± SD of three independent experiments. , indicates statistical significance. Data were analyzed using the Student t-test.