Downregulation of PTPRK Promotes Cell Proliferation and Metastasis of NSCLC by Enhancing STAT3 Activation

Figure 2

PTPRK knockdown promotes the cell proliferation, migration, and invasion ability in H1299 and A549 cells. (a) Western blotting analysis protein H1299 and A549 cells transfected two chemically synthesized siRNAs. (b) Quantitative analysis of PTPRK protein levels was calibrated with beta-actin levels of each sample from (a). (c) Representative micrographs of wound healing assay of the H1299 and A549 cells transfected with PTPRK siRNA#2 or NC. Wound closures were photographed at 0 h and 20 h after wounding. (d) Representative micrographs of Transwell invasion assay of the H1299 and A549 cells transfected with PTPRK siRNA#2 or NC. (e) Quantification of indicated invading cells in five random fields analyzed by the Transwell assays. Values represent the from three independent measurements. (f) Cell proliferation assays. H1299 and A549 cells were transfected with PTPRK siRNA#2 or NC. Cells were counted by a CCK-8 kit after 6 h, 24 h, 48 h, and 72 h. Values represent the from three independent measurements.

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