Localized Amyloidosis of the Upper Aerodigestive Tract: Complex Analysis of the Cellular Infiltrate and the Amyloid Mass

Table 3

Summary of the nano-HPLC-MS(MS) results. Percents of total immunoglobulin, Ig κ chain constant region, Ig λ chain constant region, and κ/(κ+λ) ratio were determined by label-free quantitation and normalized to total amyloid mass. The κ/(κ+λ) ratio of the cases was determined by nano-HPLC-MS(MS) using label-free quantitation. Due to the presence of low abundance Ig components, data were normalized to total amyloid mass, resulting in small numerical differences compared to Figure 2.

	Age/gender	Anatomic site	Nano-HPLC-MS(MS) total immunoglobulin (%)	Nano-HPLC-MS(MS) κ chain (%)	Nano-HPLC-MS(MS) λ chain (%)	Nano-HPLC-MS(MS) κ/(κ+λ)
1	61, male	Larynx (vocal+ventricular fold)	2.82%	0.41%	1.16%	0.26
2	44, male	Epipharynx	1.99%	0.39%	0.95%	0.29
3	51, female	Larynx (vocal fold+supraglottis)	3.34%	0.40%	2.84%	0.12
4.	62, male	Oral cavity (palate)	9.28%	6.89%	0.40%	0.94
5.	31, male	Larynx (ventricular fold, right)	6.12%	0.10%	4.63%	0.02